Flow Cytometry of anti-CD80 FITC - 200-B02-N89

Datasheet

CD80 Fluorescein Antibody

Name: CD80 Fluorescein Antibody
Brand: Rockland Immunochemicals
Price: 365.0 USD
Availability: OutOfStock

Hamster Armenian Monoclonal 16-10A1 IgG

Item No. 200-B02-N89

Size: 500 µg

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Mouse

Host Species: Hamster Armenian

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Specifications for CD80 Fluorescein Antibody

Product Details

Description: Anti-CD80 (Armenian Hamster) Fluorescein Conjugated Monoclonal Antibody - 200-B02-N89

Synonyms: T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7, CD80, Cd80

Host Species: Hamster Armenian

Conjugate: Fluorescein (FITC)

Clonality: Monoclonal

Format: IgG

Target Details

Gene Name: Cd80 - View All Cd80 Products

Reactivity: Mouse

Immunogen: Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CD80 antigen.

Purity/Specificity: Fluorescein conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD80. Cross reactivity with CD80 from other sources has not been tested. Anti-CD80 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Database Links:

Q00609 - UniProtKB

NP_033985.3 - NCBI Protein

12519 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD80 is tested for FLOW and useful for Immunohistochemistry, Immunofluorescence, and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: 0.5mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.1% Gelatin

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Expiration: Expiration date is six (6) months from date of receipt.

Background

Anti-CD80 antibody reacts with mouse CD80, also known as B7-1, a 55 kDa type I transmembrane protein ligand for CD152 (CTLA-4) and for CD28, a co-stimulatory receptor for the T cell receptor (TCR). CD28 also binds a second B7 ligand known as CD86 (B7-2). Both CD80 and CD86 are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 or CTLA-4 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.