Flow Cytometry of anti-CD80 APC - 200-B26-N89

Datasheet

CD80 Allophycocyanin Antibody

Name: CD80 Allophycocyanin Antibody
Brand: Rockland Immunochemicals
Price: 545.0 USD
Availability: OutOfStock

Hamster Armenian Monoclonal 16-10A1 IgG

Item No. 200-B26-N89

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Mouse

Host Species: Hamster Armenian

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Specifications for CD80 Allophycocyanin Antibody

Product Details

Description: Anti-CD80 (Armenian Hamster) Allophycocyanin Conjugated Monoclonal Antibody - 200-B26-N89

Synonyms: T-lymphocyte activation antigen CD80, Activation B7-1 antigen, B7, CD80, Cd80

Host Species: Hamster Armenian

Conjugate: Allophycocyanine (APC)

Clonality: Monoclonal

Format: IgG

Target Details

Gene Name: Cd80 - View All Cd80 Products

Reactivity: Mouse

Immunogen Type: Other

Immunogen: Anti-CD80 Antibody (Monoclonal) was produced by repeated immunizations with CHO cell line transfected with mouse B7 (CD80).

Purity/Specificity: Allophycocyanin conjugated CD80 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD80. CD80 has cross reactivity with canine; cross reactivity from other sources has not been tested. Anti-CD80 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Database Links:

Q00609 - UniProtKB

NP_033985.3 - NCBI Protein

12519 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD80 is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: 0.2mg/mL Sufficient to run approximately 100 tests

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiration: Expiration date is six (6) months from date of receipt.

Background

CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.