Flow Cytometry of Mouse anti-CD69 FITC - 200-302-N73

Datasheet

CD69 Fluorescein Antibody

Name: CD69 Fluorescein Antibody
Brand: Rockland Immunochemicals
Price: 399.0 USD
Availability: OutOfStock

Mouse Monoclonal FN50 IgG1 kappa

Item No. 200-302-N73

Size: 500 µL

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD69 Fluorescein Antibody

Product Details

Description: Anti-CD69 (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-N73

Synonyms: Early activation antigen CD69, Activation inducer molecule, AIM, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69, CLEC2C

Host Species: Mouse

Conjugate: Fluorescein (FITC)

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: CD69 - View All CD69 Products

Reactivity: Human

Immunogen: Anti-CD69 Antibody (Monoclonal) was produced by repeated immunizations with CD69 antigen.

Purity/Specificity: Fluorescein conjugated CD69 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD69. Reactivity is observed against human CD69. Cross reactivity is observed with Chimpanzee, Baboon, Cynomolgus, Rhesus, and Pigtailed Macaque. Reactivity with CD69 from other sources has not been tested. Anti-CD69 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Database Links:

Q07108 - UniProtKB

NP_001772.1 - NCBI Protein

969 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD69 is tested for Flow Cytometry is useful for Immunohistochemistry, and Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: Sufficient to run approximately 100 tests

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.2% BSA (w/v)

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Expiration: Expiration date is six (6) months from date of receipt.

Background

CD69 is a 27-33 kD type II transmembrane protein also known as activation inducer molecule (AIM), very early activation antigen (VEA), and MLR3. It is a member of the C-type lectin family, expressed as a disulfide-linked homodimer. Other members of this receptor family include NKG2, NKR-P1 CD94, and Ly49. CD69 is transiently expressed on activated leukocytes including T cells, thymocytes, B cells, NK cells, neutrophils, and eosinophils. CD69 is constitutively expressed by a subset of medullary mature thymocytes, platelets, mantle B cells, and certain CD4+ T cells in germinal centers of normal lymph nodes. CD69 is involved in early events of lymphocyte, monocyte, and platelet activation, and has a functional role in redirected lysis mediated by activated NK cells.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.