Flow Cytometry of anti-CD62L FITC - 200-502-N87

Datasheet

CD62L Fluorescein Antibody

Name: CD62L Fluorescein Antibody
Brand: Rockland Immunochemicals
Price: 399.0 USD
Availability: OutOfStock

Rat Monoclonal MEL-14 IgG2a kappa

Item No. 200-502-N87

Size: 500 µg

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Mouse

Host Species: Rat

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Specifications for CD62L Fluorescein Antibody

Product Details

Description: Anti-CD62L (RAT) Fluorescein Conjugated Monoclonal Antibody - 200-502-N87

Synonyms: L-selectin CD62 antigen-like family member L Leukocyte adhesion molecule 1 LAM-1 Leukocyte-endothelial cell adhesion molecule 1 LECAM1 Lymph node homing receptor Lymphocyte antigen 22 Ly-22 Lymphocyte surface MEL-14 antigen CD62L Sell Lnhr, Ly-22, Ly22

Host Species: Rat

Conjugate: Fluorescein (FITC)

Clonality: Monoclonal

Format: IgG2a

Target Details

Gene Name: Sell - View All Sell Products

Reactivity: Mouse

Immunogen: Anti-CD62L Antibody (Monoclonal) was produced by repeated immunizations with CD62L antigen.

Purity/Specificity: Fluorescein conjugated CD62L Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against mouse CD62L. Cross reactivity with CD62L from other sources has not been tested. Anti-CD62L is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Database Links:

P18337 - UniProtKB

NP_035476.1 - NCBI Protein

20343 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD62L is tested for FLOW and useful for Immunohistochemistry and Immunoprecipitation using mouse spleen cells, or an appropriate cell type (where indicated). Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: 0.5mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.1% Gelatin

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Expiration: Expiration date is six (6) months from date of receipt.

Background

Anti-CD62L antibody is specific for mouse CD62L, also known as L-Selectin, a cell adhesion molecule which facilitates lymphocyte rolling on activated vascular endothelium and homing to high endothelial venules (HEV) as immune cells transmigrate from blood into peripheral tissues. L-Selectin is a member of a family of Selectin molecules which act together with the integrin family of adhesion molecules to mediate leukocyte-endothelial interactions. L-Selectin is characteristically expressed by neutrophils, and is also found on B cells, monocytes, granulocytes, and at varying levels on naive, effector and memory T cells. It is rapidly shed upon cell activation, releasing into the circulation a soluble form whose biological role is of particular interest in cancer biology research.The MEL-14 antibody may be used as a phenotypic marker for CD62L expression on a variety of immune cell types. Please note that CD62L (L-Selectin) itself is also referred to as MEL-14 in the literature.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.