Flow Cytometry - Mouse anti-HUMAN CD47 PE

Datasheet

CD47 Phycoerythrin Antibody

Name: CD47 Phycoerythrin Antibody
Brand: Rockland Immunochemicals
Price: 645.0 USD
Availability: OutOfStock

Mouse Monoclonal B6H12.2 IgG1

Item No. 200-308-M68

Size: 1 Each

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD47 Phycoerythrin Antibody

Product Details

Description: Anti-CD47 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-M68

Synonyms: Leukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, IAP, Protein MER6, CD47

Host Species: Mouse

Conjugate: R-Phycoerythrin (RPE)

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: CD47 - View All CD47 Products

Reactivity: Human

Immunogen: Anti-CD47 Antibody (Monoclonal) was produced by repeated immunizations with CD47 antigen.

Purity/Specificity: Phycoerythrin conjugated CD47 Monoclonal Antibody was Protein G Purified and is directed against human CD47. Reactivity is observed against human CD47. Cross reactivity with CD47 from other sources has not been tested.

Database Links:

Q08722 - UniProtKB

NP_001768.1 - NCBI Protein

961 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD47 is tested for Flow Cytometry (Cell Surface). Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: 0.80 mg/mL Sufficient to run approximately 100 tests

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.05% (w/v) Sodium Azide

Stabilizer: 0.05% BSA

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiration: Expiration date is six (6) months from date of receipt.

Background

CD47, originally named integrin-associated protein (IAP), is a 50-kDa protein containing five membrane spanning sequences and a short cytoplasmic tail. CD47 appears to be important in myeloid cell activation and migration across endothelial and epithelial monolayers. Anti-CD47 antibody has been shown to inhibit polymorphonuclear neutrophil (PMN) transmigration across cell monolayers and matrix. CD47 and its ligands, thrombospondin 1 (TSP-1) and SIRP-a, are important regulators of dendritic cells. Recently, it has been shown that CD47 and TLR2 cross-talk to regulate PMN transmigration. The activation of TLR2/6 by MALP-2, a TLR2 specific agonist, potently inhibits human and murine PMN transmigration. The cross-talk between TLR2 and CD47 may be mediated through MYD88.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.