Flow Cytometry of Mouse anti-CD45RO APC - 200-326-N71

Datasheet

CD45RO Allophycocyanin Antibody

Name: CD45RO Allophycocyanin Antibody
Brand: Rockland Immunochemicals
Price: 495.0 USD
Availability: OutOfStock

Mouse Monoclonal UCHL1 IgG2a kappa

Item No. 200-326-N71

Size: 500 µL

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD45RO Allophycocyanin Antibody

Product Details

Description: Anti-CD45RO (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N71

Synonyms: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, PTPRC , CD45

Host Species: Mouse

Conjugate: Allophycocyanine (APC)

Clonality: Monoclonal

Format: IgG2a

Target Details

Gene Name: PTPRC - View All PTPRC Products

Reactivity: Human

Immunogen: Anti-CD45RO Antibody (Monoclonal) was produced by repeated immunizations with CD45RO antigen.

Purity/Specificity: Allophycocyanin conjugated CD45RO Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD45RO. Reactivity is observed against human CD45RO. Cross reactivity is observed with Chimpanzee. Reactivity with CD45RO from other sources has not been tested. Anti-CD45RO is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Database Links:

P08575 - UniProtKB

NP_002829.3 - NCBI Protein

5788 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD45RO is tested for Flow Cytometry and is useful in Immunoprecipitation, Immunohistochemistry, Immunofluorescence, and Western Blot. Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: 0.05 mg/mL Sufficient to run approximately 100 tests

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.1% Gelatin

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiration: Expiration date is six (6) months from date of receipt.

Background

The UCHL1 antibody reacts with the human CD45 isoform known as CD45RO, a protein tyrosine phosphatase of 220 kDa. CD45 is one of the most abundant hematopoietic markers, and is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). Various isoforms are generated and expressed in cell-specific patterns. With their broad cell distribution, CD45 isoforms are critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, and others. The UCHL1 antibody is widely used as a marker for human CD45RO expression on thymocytes, activated memory T cells, monocytes, macrophages and granulocytes. The antibody is reported to be cross-reactive for Chimpanzee CD45RO.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.