Flow Cytometry of Mouse anti-CD45RA APC - 200-326-N70

Datasheet

CD45RA Allophycocyanin Antibody

Name: CD45RA Allophycocyanin Antibody
Brand: Rockland Immunochemicals
Price: 585.0 USD
Availability: OutOfStock

Mouse Monoclonal HI100 IgG2b kappa

Item No. 200-326-N70

Size: 500 µL

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD45RA Allophycocyanin Antibody

Product Details

Description: Anti-CD45RA (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N70

Synonyms: CD9 antigen, 5H9 antigen, Cell growth-inhibiting gene 2 protein, Leukocyte antigen MIC3, Motility-related protein, MRP-1, Tetraspanin-29, Tspan-29, p24, CD9, MIC3, TSPAN29, GIG2

Host Species: Mouse

Conjugate: Allophycocyanine (APC)

Clonality: Monoclonal

Format: IgG2b

Target Details

Gene Name: CD9 - View All CD9 Products

Reactivity: Human

Immunogen: Anti-CD45RA Antibody (Monoclonal) was produced by repeated immunizations with CD45RA antigen.

Purity/Specificity: Allophycocyanin conjugated CD45RA Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD45RA. Reactivity is observed against human CD45RA. Cross reactivity is observed with Chimpanzee. Reactivity with CD45RA from other sources has not been tested. Anti-CD45RA is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Database Links:

P21926 - UniProtKB

NP_001760.1 - NCBI Protein

928 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD45RA is tested for Flow Cytometry and is useful in Immunohistochemistry and Western Blot. Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: Sufficient to run approximately 100 tests

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.2% BSA (w/v)

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiration: Expiration date is six (6) months from date of receipt.

Background

CD45RA is a 205-220 kD single chain type I glycoprotein. It is an exon 4 splice variant of the tyrosine phosphatase CD45. The CD45RA isoform is expressed on resting/naïve T cells, medullary thymocytes, B cells and monocytes. CD45RA enhances both T cell receptor and B cell receptor signaling. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4. CD45 has also been reported to bind galectin-1. CD45 isoform expression can change in response to cytokines.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.