Flow Cytometry of Mouse anti-CD45 PE - 200-308-N68

Datasheet

CD45 Phycoerythrin Antibody

Name: CD45 Phycoerythrin Antibody
Brand: Rockland Immunochemicals
Price: 325.0 USD
Availability: OutOfStock

Mouse Monoclonal HI30 IgG1 kappa

Item No. 200-308-N68

Size: 500 µL

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD45 Phycoerythrin Antibody

Product Details

Description: Anti-CD45 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N68

Synonyms: Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, CD45, PTPRC

Host Species: Mouse

Conjugate: R-Phycoerythrin (RPE)

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: PTPRC - View All PTPRC Products

Reactivity: Human

Immunogen: Anti-CD45 Antibody (Monoclonal) was produced by repeated immunizations with CD45 antigen.

Purity/Specificity: Phycoerythrin conjugated CD45 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD45. Reactivity is observed against human CD45. Cross reactivity is observed with Chimpanzee. Reactivity with CD45 from other sources has not been tested. Anti-CD45 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.

Database Links:

P08575 - UniProtKB

NP_002829.3 - NCBI Protein

5788 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD45 is tested for Flow Cytometry and is useful for Immunohistochemistry and Western Blotting. Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: Sufficient to run approximately 100 tests

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.2% BSA (w/v)

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiration: Expiration date is six (6) months from date of receipt.

Background

CD45 is a 180-240 kD single chain type I membrane glycoprotein also known as leukocyte common antigen (LCA) and T200. It is a tyrosine phosphatase expressed on the plasma membrane of all hematopoietic cells, except erythrocytes and platelets. CD45 is a signaling molecule that regulates a variety of cellular processes including cell growth, differentiation, cell cycle, and oncogenic transformation. CD45 plays a critical role in T and B cell antigen receptor-mediated activation by dephosphorylating substrates including p56Lck, p59Fyn, and other Src family kinases. CD45 non-covalently associates with lymphocyte phosphatase-associated phosphoprotein (LPAP) on T and B lymphocytes. CD45 has been reported to bind galectin-1 and to be associated with several other cell surface antigens including CD1, CD2, CD3, and CD4.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.