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CD45 Fluorescein Antibody
Mouse Monoclonal HI30 IgG1 kappa
1 References
200-302-N68
500 µL
Liquid (sterile filtered)
FC
Human
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-CD45 (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-N68
Receptor-type tyrosine-protein phosphatase C, Leukocyte common antigen, CD45, PTPRC
Mouse
Fluorescein (FITC)
Monoclonal
IgG1
Target Details
PTPRC - View All PTPRC Products
Human
Anti-CD45 Antibody (Monoclonal) was produced by repeated immunizations with CD45 antigen.
Fluorescein conjugated CD45 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD45. Reactivity is observed against human CD45. Cross reactivity is observed with Chimpanzee. Reactivity with CD45 from other sources has not been tested. Anti-CD45 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.
Application Details
FC
Anti-CD45 is tested for Flow Cytometry and is useful for Immunoprecipitation, Immunohistochemistry, and Western Blot. Researchers should determine optimal titers for applications that are not stated.
Formulation
0.05 mg/mL Sufficient to run approximately 100 tests
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.09% (w/v) Sodium Azide
0.1% Gelatin
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.
Expiration date is six (6) months from date of receipt.
The HI30 antibody reacts with human CD45, one of the most abundant hematopoietic markers and one that is expressed on all leukocytes (the Leukocyte Common Antigen, LCA). CD45 is a protein tyrosine phosphatase existing in several isoforms, each being generated and expressed in cell-specific patterns. With its broad cell distribution, CD45 is critical for many leukocyte functions, regulating signal transduction and cell activation associated with the T cell receptor, B cell receptor, and IL-2 receptor. Other forms of CD45, with restricted cellular expression, include CD45R (B220), CD45RA, CD45RB, CD45RO and others. The HI30 antibody is widely used as a marker for human CD45 expression on T cells, B cells, monocytes, macrophages, and NK cells.
Guo X. et al. (2020). Effects of Ginkgo biloba on Early Decompression after Spinal Cord Injury. Evid Based Complement Alternat Med.
Applications
FC, FACS, FLOW
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
