Flow Cytometry of Mouse anti-CD40 APC - 200-326-N67

Datasheet

CD40 Allophycocyanin Antibody

Name: CD40 Allophycocyanin Antibody
Brand: Rockland Immunochemicals
Price: 609.0 USD
Availability: OutOfStock

Mouse Monoclonal 5C3 IgG1 kappa

Item No. 200-326-N67

Size: 500 µL

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD40 Allophycocyanin Antibody

Product Details

Description: Anti-CD40 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N67

Synonyms: BP50, TNFRSF5, Tumor necrosis factor receptor superfamily member 5, B-cell surface antigen CD40, Bp50, CD40L receptor, CDw40

Host Species: Mouse

Conjugate: Allophycocyanine (APC)

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: CD40 - View All CD40 Products

Reactivity: Human

Immunogen: Anti-CD40 Antibody (Monoclonal) was produced by repeated immunizations with CD40 antigen.

Purity/Specificity: Allophycocyanin conjugated CD40 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD40. Reactivity is observed against human CD40. Cross reactivity is observed with Baboon, Chimpanzee, Cynomolgus Monkey, Rhesus, and Squirrel Monkey. Reactivity with CD40 from other sources has not been tested. Anti-CD40 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Database Links:

P25942 - UniProtKB

NP_001241.1 - NCBI Protein

958 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD40 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: sufficient to run 100 tests Sufficient to run approximately 100 tests

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.2% BSA (w/v)

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiration: Expiration date is six (6) months from date of receipt.

Background

CD40 is a 48 kD type I glycoprotein also known as BP50. It is a member of the TNFR superfamily primarily expressed on B cells, macrophages, follicular dendritic cells, endothelial cells, fibroblasts, and at low levels on plasma cells. CD40 has been reported to be involved in B cell differentiation, costimulation, isotype class-switching, and protection of B cells from apoptosis. Additionally, CD40 is important for T cell-B cell interactions. The ligand of CD40 is CD154 (CD40 ligand). The 5C3 antibody has been reported to promote B cell proliferation in the presence of anti-IgM, IL-4 or PMA, partially blocking CD40 binding to CD40L, and B cells rescue from apoptosis.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.