Datasheet

CD4 Fluorescein Antibody

Name: CD4 Fluorescein Antibody
Brand: Rockland Immunochemicals
Price: 242.0 USD
Availability: OutOfStock

Mouse Monoclonal OKT4 IgG2b kappa

Item No. 200-302-N18

Size: 500 µL

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD4 Fluorescein Antibody

Product Details

Description: Anti-CD4 (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-N18

Synonyms: T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, FITC anti-human CD4, RPA-T4, FITC, T4

Host Species: Mouse

Conjugate: Fluorescein (FITC)

Clonality: Monoclonal

Format: IgG2b

Target Details

Gene Name: CD4 - View All CD4 Products

Reactivity: Human

Immunogen: Anti-CD4 Antibody (Monoclonal) was produced by repeated immunizations with CD4 antigen.

Purity/Specificity: Fluorescein conjugated CD4 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD4. Reactivity is observed against human CD4, Cynomolgus, Rhesus, and chimpanzee. This antibody recognizes a different epitope, and thus does not block binding of clone RPA-T4. Cross reactivity with CD4 from other sources has not been tested. Anti-CD4 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Database Links:

P01730 - UniProtKB

NP_000607.1 - NCBI Protein

920 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD4 has been tested in Flow Cytometry and is useful for Immunoprecipitation, Immunofluorescence, and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: 0.05mg/mL Sufficient to run approximately 100 tests

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.1% Gelatin

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Expiration: Expiration date is six (6) months from date of receipt.

Background

The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells.The OKT4 antibody is widely used as a phenotypic marker for CD4 expression.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.