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CD4 Allophycocyanin Antibody
Mouse Monoclonal OKT4 IgG2b kappa
200-326-N18
500 µL
Liquid (sterile filtered)
FC
Human
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-CD4 (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N18
T-cell surface glycoprotein CD4, T-cell surface antigen T4/Leu-3, CD4, FITC anti-human CD4, RPA-T4, APC, T4
Mouse
Allophycocyanine (APC)
Monoclonal
IgG2b
Target Details
CD4 - View All CD4 Products
Human
Anti-CD4 Antibody (Monoclonal) was produced by repeated immunizations with CD4 antigen.
Allophycocyanin conjugated CD4 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD4. Reactivity is observed against human CD4, Cynomolgus, Rhesus, and chimpanzee. This antibody recognizes a different epitope, and thus does not block binding of clone RPA-T4. Cross reactivity with CD4 from other sources has not been tested. Anti-CD4 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.
Application Details
FC
Anti-CD4 is tested in Flow Cytometry and useful for Immunoprecipitation, Immunofluorescence, Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.
Formulation
0.025 mg/mL Sufficient to run approximately 100 tests
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.09% (w/v) Sodium Azide
0.1% Gelatin
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Expiration date is six (6) months from date of receipt.
The OKT4 antibody reacts with human CD4, a 59 kDa protein which acts as a co-receptor for the T cell receptor (TCR) in its interaction with MHC Class II molecules on antigen-presenting cells. The extracellular domain of CD4 binds to the beta-2 domain of MHC Class II, while its cytoplasmic tail provides a binding site for the tyrosine kinase lck, facilitating the signaling cascade that initiates T cell activation. CD4, and co-receptors CCR5 and CXCR4, may also be utilized by HIV-1 to enter T cells. Human CD4 is typically expressed on thymocytes, some mature T cell populations such as Th17 and T regulatory (Treg) cells, as well as on dendritic cells. The OKT4 antibody is widely used as a phenotypic marker for CD4 expression.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
