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CD3 Phycoerythrin Antibody
Mouse Monoclonal HIT3a IgG2a kappa
200-308-N12
500 µL
Liquid (sterile filtered)
FC
Human
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-CD3 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N12
T-cell surface glycoprotein CD3 epsilon chain, T-cell surface antigen T3/Leu-4 epsilon chain, CD3e
Mouse
R-Phycoerythrin (RPE)
Monoclonal
IgG2a
Target Details
CD3E - View All CD3E Products
Human
Anti-CD3 Antibody (Monoclonal) was produced by repeated immunizations with CD3 antigen.
Phycoerythrin conjugated CD3 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD3. Reactivity is observed against human CD3. Cross reactivity with CD3 from other sources has not been tested. Anti-CD3 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.
Application Details
FC
Anti-CD3 is tested for Flow Cytometry. Researchers should determine optimal titers for applications that are not stated.
Formulation
0.05mg/mL Sufficient to run approximately 100 tests
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.09% (w/v) Sodium Azide
0.1% Gelatin
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Expiration date is six (6) months from date of receipt.
The Hit3a antibody is specific for human CD3e, also known as CD3 epsilon, a 20 kDa subunit of the T cell receptor complex, along with CD3 gamma and CD3 delta. These integral membrane protein chains assemble with additional chains of the T cell receptor (TCR), as well as CD3 zeta chain, to form the T cell receptor – CD3 complex. Together with co-receptors CD4 or CD8, the complex serves to recognize antigens bound to MHC molecules on antigen-presenting cells. These interactions promote T cell receptor signaling (T cell activation), inducing cell proliferation, differentiation, production of cytokines or activation-induced cell death. CD3 is differentially expressed during thymocyte-to-T cell development and on all mature T cells. The Hit3a antibody is a widely used phenotypic marker for human T cells. In addition, binding/cross-linking of Hit3a antibody to CD3e can induce cell activation. The antibody has also been demonstrated to be cross-reactive with Chimpanzee CD3.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
