Datasheet

CD28 Fluorescein Antibody

Name: CD28 Fluorescein Antibody
Brand: Rockland Immunochemicals
Price: 325.0 USD
Availability: OutOfStock

Mouse Monoclonal CD28.2 IgG1 kappa

Item No. 200-302-N66

Size: 500 µL

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD28 Fluorescein Antibody

Product Details

Description: Anti-CD28 (MOUSE) Fluorescein Conjugated Monoclonal Antibody - 200-302-N66

Synonyms: T-cell-specific surface glycoprotein CD28, TP44, CD28

Host Species: Mouse

Conjugate: Fluorescein (FITC)

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: CD28 - View All CD28 Products

Reactivity: Human

Immunogen: Anti-CD28 Antibody (Monoclonal) was produced by repeated immunizations with CD28 antigen.

Purity/Specificity: Fluorescein conjugated CD28 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD28. Reactivity is observed against human CD28, Baboon, Chimpanzee, Cynomolgus, and Rhesus. Cross reactivity with CD28 from other sources has not been tested. Anti-CD28 is conjugated with FITC under optimal conditions and the solution is free of unconjugated FITC.

Database Links:

P10747 - UniProtKB

NP_001230006.1 - NCBI Protein

940 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD28 is tested for Flow Cytometry and is useful in Immunoprecipitation and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: 0.1 mg/mL Sufficient to run approximately 100 tests

Buffer: 0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.1% Gelatin

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. DO NOT FREEZE. This product is light sensitive.

Expiration: Expiration date is six (6) months from date of receipt.

Background

The CD28 antibody reacts with human CD28, a 44 kDa type I surface glycoprotein which acts as a co-stimulatory receptor in support of the T cell receptor (TCR). CD28 exists as a homodimer with specificity for two known ligands, known as B7-1 (CD80) and B7-2 (CD86), which are expressed on activated B cells and antigen-presenting cells. These ligands trigger CD28 signaling in concert with TCR activation to drive T cell proliferation, induce high-level expression of IL-2, impart resistance to apoptosis, and enhance T cell cytotoxicity. The interaction / co-stimulatory signaling between the B7 ligands and CD28 provides crucial communication between T cells and B cells or APCs to coordinate the adaptive immune response. Other members of the CD28 family of receptors include CTLA-4 (CD152), PD-1 (CD279), ICOS and BTLA. The CD28.2 antibody may be used as a phenotypic marker for human CD28, expressed on all CD4+ T cells and CD8+ T cells, and is widely used as a reagent for activation of the CD28 receptor in vitro and in vivo.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.