Datasheet

CD20 Phycoerythrin Antibody

Name: CD20 Phycoerythrin Antibody
Brand: Rockland Immunochemicals
Price: 425.0 USD
Availability: OutOfStock

Mouse Monoclonal 2H7 IgG2b kappa

Item No. 200-308-N64

Size: 500 µL

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD20 Phycoerythrin Antibody

Product Details

Description: Anti-CD20 (MOUSE) Phycoerythrin Conjugated Monoclonal Antibody - 200-308-N64

Synonyms: B-lymphocyte antigen CD20, B-lymphocyte surface antigen B1, Bp35, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1, CD20

Host Species: Mouse

Conjugate: R-Phycoerythrin (RPE)

Clonality: Monoclonal

Format: IgG2b

Target Details

Gene Name: MS4A1 - View All MS4A1 Products

Reactivity: Human

Immunogen: Anti-CD20 Antibody (Monoclonal) was produced by repeated immunizations with CD20 antigen.

Purity/Specificity: Phycoerythrin conjugated CD20 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD20. Reactivity is observed against human CD20 Chimpanzee, Baboon, Cynomolgus, Rhesus, Pigtailed Macaque, Capuchin Monkey, and Squirrel Monkey. Cross reactivity with CD20 from other sources has not been tested. Anti-CD20 is conjugated with PE under optimal conditions and the solution is free of unconjugated PE.

Database Links:

P11836 - UniProtKB

NP_068769.2 - NCBI Protein

931 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD20 is tested for Flow Cytometry and is useful in Immunoprecipitation and Immunohistochemistry. Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: Sufficient to run approximately 100 tests

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.2% BSA (w/v)

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiration: Expiration date is six (6) months from date of receipt.

Background

CD20 is a 33-37 kD, four transmembrane spanning protein, also known as B1 and Bp35. CD20 is expressed on pre-B-cells, resting and activated B cells (not plasma cells), some follicular dendritic cells, and at low levels on a T cell subset. CD20 is heavily phosphorylated on activated B cells and malignant B cells. Homo-oligomeric complexes of CD20 are thought to form Ca2+ conductive ion channels in the plasma membrane of B cells. The CD20 molecule is involved in B-cell activation and is associated with various Src family kinases (Lyn, Lck, Fyn). It exists in a complex with MHC class I and II, CD53, CD81, and CD82.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.