Flow Cytometry of Mouse anti-CD154 Allophycocyanin

Datasheet

CD154 (CD40L) Allophycocyanin Antibody

Name: CD154 (CD40L) Allophycocyanin Antibody
Brand: Rockland Immunochemicals
Price: 655.0 USD
Availability: OutOfStock

Mouse Monoclonal Clone 24-31 IgG1 kappa

Item No. 200-326-N76

Size: 500 µL

Physical State: Liquid (sterile filtered)

Applications: FC

Reactivity: Human

Host Species: Mouse

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Specifications for CD154 (CD40L) Allophycocyanin Antibody

Product Details

Description: Anti-CD154 (CD40L) (MOUSE) Allophycocyanin Conjugated Monoclonal Antibody - 200-326-N76

Synonyms: CD40 ligand, CD40-L, T-cell antigen Gp39, TNF-related activation protein, TRAP, Tumor necrosis factor ligand superfamily member 5, CD40LG, CD40L, TNFSF5, TRAP

Host Species: Mouse

Conjugate: Allophycocyanine (APC)

Clonality: Monoclonal

Format: IgG1

Target Details

Gene Name: CD40LG - View All CD40LG Products

Reactivity: Human

Immunogen: Anti-CD154 Antibody (Monoclonal) was produced by repeated immunizations with CD154 antigen.

Purity/Specificity: Allophycocyanin conjugated CD154 Monoclonal Antibody was purified from tissue culture supernatant via affinity chromatography and is directed against human CD154. Reactivity is observed against human CD154. Cross reactivity is observed with Cynomolgus and Rhesus. Reactivity with CD154 from other sources has not been tested. Anti-CD154 is conjugated with APC under optimal conditions and the solution is free of unconjugated APC.

Database Links:

P29965 - UniProtKB

NP_000065.1 - NCBI Protein

959 - Gene ID

Application Details

Tested Applications: FC

Application Note: Anti-CD154 is tested for Flow Cytometry and useful in Immunofluorescence. Researchers should determine optimal titers for applications that are not stated.

Formulation

Concentration: Sufficient to run approximately 100 tests

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.09% (w/v) Sodium Azide

Stabilizer: 0.2% BSA (w/v)

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.

Expiration: Expiration date is six (6) months from date of receipt.

Background

CD154 (CD40 ligand) is also known as CD40L, gp39, TRAP and T-BAM. CD40 ligand is a 32-39 kD type II transmembrane glycoprotein. It is a member of the TNF superfamily and is expressed on activated T cells. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Additionally, binding of CD40L to CD40 on B cells promotes the secretion of immunoglobulin and Ig isotype switching. CD40L is also involved in the regulation of cytokine production by T cells.

Related Protocols

Flow Cytometry (FC) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.