Datasheet

CCNT1 Antibody

Name: CCNT1 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-AG1

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, ELISA, IHC, IF

Reactivity: Human, Mouse, Rat

Host Species: Rabbit

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Specifications for CCNT1 Antibody

Product Details

Description: Anti-CCNT1 (RABBIT) Antibody - 600-401-AG1

Synonyms: rabbit anti-cyclin T1 antibody, CYCT 1 antibody, cycT1 antibody, HIVE1 antibody, Human immunodeficiency virus 1 expression antibody, CCNT1 Antibody, CCNT, CYCT1, HIVE1, Cyclin-T1, CycT1

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: CCNT1 - View All CCNT1 Products

Reactivity: Human, Mouse, Rat

Immunogen Type: Conjugated Peptide

Immunogen: Anti- CCNT1 antibody was prepared from whole rabbit serum produced by repeated immunizations with an 18 amino acid peptide near the C-terminus of human CCNT1.

Purity/Specificity: Anti-CCNT1 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Cross reactivity with CCNT1 from other sources has not been determined.

Database Links:

O60563 - UniProtKB

NP_001231 - NCBI Protein

904 - Gene ID

Application Details

Tested Applications: ELISA, IF, IHC, WB

Application Note: Anti-CCNT1 Antibody has been tested for use in ELISA, Western Blotting, Immunohistochemistry and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 81 kDa in Western Blots of specific cell lysates and tissues.

Formulation

Concentration: 1 mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Preservative: 0.02% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Cyclins function as regulators of CDK kinases and exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. The cyclin-T1 protein (CCNT1) belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. CCNT1 tightly associates with CDK9 kinase, and was found to be a major subunit of the transcription elongation factor p-TEFb. The kinase complex containing CCNT1 and the elongation factor can interact with, and act as a cofactor of human immunodeficiency virus type 1 (HIV-1) Tat protein, and was shown to be both necessary and sufficient for full activation of viral transcription. CCNT1 and its kinase partner were also found to be involved in the phosphorylation and regulation of the carboxy-terminal domain (CTD) of the largest RNA polymerase II subunit.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Immunohistochemistry (IHC) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Sandwich ELISA Protocol for Collagen

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.