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CASZ1 Antibody
Rabbit Polyclonal
5 References
600-401-B62S
600-401-B62
25 µL
100 µg
Liquid (sterile filtered)
Liquid (sterile filtered)
WB, ELISA, IHC, IF, ChIP, Multiplex
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-CASZ1 (RABBIT) Antibody - 600-401-B62
rabbit anti-CASZ1 Antibody, CASZ1, Zinc finger protein castor homolog 1, Castor-related protein, Zinc finger protein 693, CST, SRG, ZNF693
Rabbit
Polyclonal
IgG
Target Details
CASZ1 - View All CASZ1 Products
Human
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region of Human Casz1 protein.
This affinity purified antibody is directed against human CASZ1 protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with CASZ1 proteins from human, mouse, Drosophila, chimpanzee, and macaque based on a 100% homology. Partial reactivity is expected with horse and dog CASZ1 based on a 92% homology with the immunizing sequence. Cross-reactivity with CASZ1 from other sources has not been determined.
Application Details
ELISA, WB
ChIP, IF, IHC, Multiplex
- View References
This antibody has been tested for use in ELISA, IHC, IF, and western blotting. This antiserum detects endogenous CASZ1 proteins, both the hCASZ5 and hCASZ11 isoforms. Expect a band approximately 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in size corresponding CASZ1 by western blotting in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.07 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. The human castor zinc finger 1 gene (CASZ1) regulates the production of the castor transcription factor, having increased expression when cells of neural and mesenchymal origin are induced to differentiation. CASZ1 is expressed in a number of human tumors and it localizes to a genomic region frequently lost in tumors of neuroectodermal origin. The CASZ1 gene generates 2 mRNA isoforms hCASZ5 and hCASZ11 that could encode proteins containing 5 or 11 zinc finger motifs, respectively. The proteins may be especially labile. Expect a band at 125 kDa for hCASZ5 and 190 kDa for hCASZ11 in fresh lysates.
Liu, Z., Zhang, X., Lei, H. et al. (2020). CASZ1 induces skeletal muscle and rhabdomyosarcoma differentiation through a feed-forward loop with MYOD and MYOG. Nat Commun
Applications
WB, IB, PCA; ChIP
Lunde, A et al. (2019). Molecular Profiling Defines Evolutionarily Conserved Transcription Factor Signatures of Major Vestibulospinal Neuron Groups. ENeuro
Applications
IHC, ICC, Histology; IF, Confocal Microscopy
Liu et al. (2017). Identification of CASZ1 nuclear export signal (NES) reveals potential mechanisms for loss of CASZ1 tumor suppressor activity in neuroblastoma. Oncogene
Applications
IF, Confocal Microscopy; IHC, ICC, Histology
Zhang J, et al. (2013). SFMBT1 functions with LSD1 to regulate expression of canonical histone genes and chromatin-related factors. Genes Dev
Applications
WB, IB, PCA
Z Liu, A Naranjo, and CJ Thiele. (2011). CASZ1b, the Short Isoform of CASZ1 Gene, Coexpresses with CASZ1a during Neurogenesis and Suppresses Neuroblastoma Cell Growth. PLOS.ONE.
Applications
IF, Confocal Microscopy; WB, IB, PCA; Multiplex Assay
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.