Immunofluorescence of Caspase-9 Antibody

Immunocytochemistry of Caspase-9 Antibody

Datasheet

Caspase-9 Antibody

Name: Caspase-9 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-AE4

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: WB, ELISA, IHC, IF, IP

Reactivity: Human

Host Species: Rabbit

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Specifications for Caspase-9 Antibody

Product Details

Description: Anti-Caspase-9 (RABBIT) Antibody - 600-401-AE4

Synonyms: Caspase-9 Antibody, MCH6, APAF3, APAF-3, PPP1R56, ICE-LAP6, MCH6, Caspase-9, Apoptotic protease Mch-6, CASP-9

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: CASP9 - View All CASP9 Products

Reactivity: Human

Immunogen Type: Conjugated Peptide

Immunogen: Anti-Caspase-9 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 20 amino acid peptide near the internal region of human Caspase-9.

Purity/Specificity: Anti-Caspase-9 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. It has no cross response to other members in caspase family.

Database Links:

P55211 - UniProtKB

NP_001220.2 - NCBI Protein

842 - Gene ID

Application Details

Tested Applications: ELISA, IF, IHC, IP, WB

Application Note: Anti-Caspase-9 Antibody has been tested for use in ELISA, Western Blotting, Immunocytochemistry, Immunofluorescence, and Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 46 kDa in Western Blots of specific cell lysates and tissues.

Formulation

Concentration: 1 mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Preservative: 0.02% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. A novel member in the caspase family was recently identified and designated ICE-LAP6, Mch6, and Apaf-3. Caspase-9 and Apaf-1 bind to each other, which leads to caspase-9 activation. Caspase-9 is also activated by granzyme B and CPP32. Activated caspase-9 cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis. Caspase-9 play a central role in cell death induced by a wide variety of apoptosis activators including TNFa, TRAIL, anti-CD-95, FADD, and TRADD. Caspase-9 is expressed in a variety of human tissues.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

Immunohistochemistry (IHC) Protocol

Immunoprecipitation (IP) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Sandwich ELISA Protocol for Collagen

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.