Immunohistochemistry of Caspase-3 Antibody

Datasheet

Caspase-3 Antibody

Name: Caspase-3 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: OutOfStock

Rabbit Polyclonal

Item No. 600-401-AD2

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: ELISA, IHC

Reactivity: Human

Host Species: Rabbit

$475.00 /Per Item

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Specifications for Caspase-3 Antibody

Product Details

Description: Anti-Caspase-3 (RABBIT) Antibody - 600-401-AD2

Synonyms: Caspase-3 Antibody, CPP32, SCA-1, CPP32B, CPP32, Caspase-3, Apopain, CASP-3

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: CASP3 - View All CASP3 Products

Reactivity: Human

Immunogen Type: Conjugated Peptide

Immunogen: Anti-Caspase-3 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 17 amino acid synthetic peptide near the internal region of human Caspase-3.

Purity/Specificity: Anti-Caspase-3 Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. At least two isoforms of Caspase-3 are known to exist.

Database Links:

P42574 - UniProtKB

NP_004337 - NCBI Protein

836 - Gene ID

Application Details

Tested Applications: ELISA, IHC

Application Note: Anti-Caspase-3 Antibody has been tested for use in ELISA and Immunohistochemistry. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 31 kDa in Western Blots of specific cell lysates and tissues.

Formulation

Concentration: 1 mg/mL by UV absorbance at 280 nm

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Preservative: 0.02% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

Caspases are a family of cysteine proteases that can be divided into the apoptotic and inflammatory caspase subfamilies. Unlike the apoptotic caspases, members of the inflammatory subfamily are generally not involved in cell death but are associated with the immune response to microbial pathogens. The apoptotic subfamily can be further divided into initiator caspases, which are activated in response to death signals, and executioner caspases, which are activated by the initiator caspases and are responsible for cleavage of cellular substrates that ultimately lead to cell death. Caspase-3 is synthesized as an inactive proenzyme that undergoes proteolytic cleavage by caspases 8, 9 and 10 to produce 2 subunits, termed p20 and p11. These subunits dimerize to form the active enzyme. Caspase-3 proteolytically cleaves and activates other proteins such as caspases 6, 7 and 9.

Related Protocols

ELISA Protocol

Heat-induced Antigen Retrieval Protocol

Immunohistochemistry (IHC) Protocol

Protease-induced Antigen Retrieval Protocol

Sandwich ELISA Protocol for Collagen

Staining Paraffin Sections by PAP Procedure

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.