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Caspase-3 Antibody
Mouse Monoclonal 31A1067 IgG1 kappa
200-301-H63
100 µg
Liquid
WB, IHC, IF, FC
Human
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Caspase-3 (MOUSE) Monoclonal Antibody - 200-301-H63
Cpp32, Caspase-3 subunit p17, Caspase-3 subunit p12, Apopain, Cysteine protease CPP32 Caspase-3, CASP-3, 3.4.22.56,
Apopain, Cysteine protease CPP32, CPP-32, Protein Yama, SREBP cleavage activity 1, SCA-1, Caspase-3 subunit p17, Caspase-3 subunit p12
Mouse
Monoclonal
IgG1
Target Details
CASP3 - View All CASP3 Products
Human
Recombinant Protein
Caspase-3 Antibody was produced in mice by repeated immunizations with a full length recombinant Caspase-3 human protein.
Anti-Caspase-3 Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Anti-Caspase-3 from Human, mouse and rat based on 100% homology with the immunizing sequence. Cross-reactivity with Anti-Caspase-3 from other sources has not been determined.
Application Details
FC, IF, IHC, WB
Anti-Caspase 3 Antibody is tested for use in WB, EM, Flow, ICC/IF, IHC-Fr, and IHC-P. Expect a band approximately 31.5 kDa on specific lysates. The antibody detects both pro Caspase-3(32 kDa) and the large subunit of the active/cleaved form (14-21 kDa) of Caspase-3. The large subunit of the cleaved form may appear as one or two or even as a stack of bands depending on the presence or absence of the Caspase-3 pro-domain. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.05% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store Caspase 3 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Caspase-3 antibody detects human Caspase-3. Caspases are a family of cysteine proteases that are key mediators of programmed cell death or apoptosis. The precursor form of all caspases is composed of a prodomain, and large and small catalytic subunits. The active forms of caspases are generated by several stimuli including ligand-receptor interactions, growth factor deprivation and inhibitors of cellular functions. All known caspases require cleavage adjacent to aspartates to liberate one large and one small subunit, which associate into a2b2 tetramer to form the active enzyme. Gene for Caspase-3 also known as Yama, CPP32, and apopain codes for a 32-kDa protein. Caspase-3 cleaves the death substrate poly (ADP-ribose) polymerase (PARP) to a specific 85 kDa form observed during apoptosis and is inhibitable by the CrmA protein. Other Caspase-3 substrates include DNA-PK, actin, GAS2, and procaspase-6, etc. Caspase-3 is activated by cleavage events at Asp-28/Ser-29 (between N-terminal pro-domain) and Asp-175/Ser-176 (between large and small subunits) to generate a large subunit of 17-kDa and a small subunit of 12-kDa. Anti-Caapase-3 is ideal for investigators involved in apoptosis, cytokines and growth factor research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
