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Carboxypeptidase Y Antibody Peroxidase Conjugated
Rabbit Polyclonal
200-403-135
200-403-135S
200-403-135-0100
20 mg
25 µL
100 µg
Lyophilized
Liquid (sterile filtered)
Lyophilized
WB, ELISA
S. cerevisiae
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Carboxypeptidase Y (RABBIT) Antibody Peroxidase Conjugated (BULK ORDER) - 200-403-135
rabbit anti-Carboxypeptidase Y antibody peroxidase conjugation, HRP conjugated rabbit anti-Carboxypeptidase Y Antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody, Carboxypeptidase C
Rabbit
Peroxidase (HRP)
Polyclonal
IgG
Target Details
PRC1 - View All PRC1 Products
S. cerevisiae
Native Protein
Carboxypeptidase Y [Baker’s Yeast]
Carboxypeptidase Y is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Carboxypeptidase Y [Baker’s Yeast]. Cross reactivity against Carboxypeptidase Y from other sources may occur but has not been specifically determined.
Application Details
ELISA, WB
Anti-Carboxypeptidase Y Peroxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 µg of Carboxypeptidase Y [Baker’s Yeast] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.
Formulation
10 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Carboxypeptidase Y is an enzyme that catalyzes the reaction of the release of a C-terminal amino acid with broad specificity. Carboxypeptidase Y is a carboxypeptidase with optimum activity at pH 4.5-6.0. It is inhibited by diisopropyl fluorophosphate.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
