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Carboxypeptidase Y Antibody
Rabbit Polyclonal
1 References
100-401-135
2 mL
Lyophilized
WB, Cellular Assay
S. cerevisiae
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Carboxypeptidase Y (RABBIT) Antibody - 100-401-135
rabbit anti-Carboxypeptidase Y antibody, Carboxypeptidase YSCY antibody, CPY1 antibody, LBC1 antibody, PRC1 antibody, Vacuolar carboxypeptidase Y antibody, YMR297W antibody
Rabbit
Polyclonal
Antiserum
Target Details
PRC1 - View All PRC1 Products
S. cerevisiae
Native Protein
Carboxypeptidase Y [Baker’s Yeast]
This product was prepared from monospecific antiserum by a delipidation and defibrination. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-rabbit serum, purified and partially purified Carboxypeptidase Y [Baker’s Yeast]. Cross reactivity against Carboxypeptidase Y from other tissues and species may occur but have not been specifically determined.
Application Details
WB
Cellular Assay
- View References
Anti-Carboxypeptidase Y has been tested by western blot and is suitable to be assayed against 1.0 µg of Carboxypeptidase Y [Baker’s Yeast] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.
Formulation
90 mg/mL by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
2.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Carboxypeptidase Y is involved in degradation of small peptides. It digests preferentially peptides containing an aliphatic or hydrophobic residue in P1' position, as well as methionine, leucine or phenylalanine in P1 position of ester substrate. Carboxypeptidase that catalyzes the release of a C-terminal amino acid with broad specificity. It is inhibited by ZPCK.
Ohashi, Y. et al. (2010). Membrane delivery to the yeast autophagosome from the Golgi-endosomal system. Molecular Biology of the Cell
Applications
Pulse-Chase
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
