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BUBR1 Kinase Antibody
Mouse Monoclonal 8G1 IgG1
1 References
200-301-902
100 µg
Liquid (sterile filtered)
WB, IF, IP, Multiplex
Human
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-BUBR1 Kinase (MOUSE) Monoclonal Antibody - 200-301-902
mouse anti-BUBR1 antibody, Beta homolog of S. cerevisiae budding uninhibited by benzimidazoles antibody, BUB1 budding uninhibited by benzimidazoles 1 homolog beta antibody, Bub1A antibody, Mitotic checkpoint serine/threonine-protein kinase BUB1 beta, Mitotic checkpoint kinase MAD3L, MAD3/BUB1-related protein kinase, hBUBR1
Mouse
Monoclonal
IgG1
Target Details
BUB1B - View All BUB1B Products
Human
Recombinant Protein
This protein A purified monoclonal antibody was produced by repeated immunizations with a recombinant protein corresponding to amino acid residues 1-350 of human BUBR1 Kinase protein.
This Protein A purified antibody is directed against human BUBR1 Kinase protein. The product was purified from tissue culture supernatant by chromatography. This antibody has only been tested on human cells. Reactivity against homologues from other sources is not known.
Application Details
IP, WB
IF, Multiplex
- View References
This protein A purified antibody has been tested for use in immunoprecipitation, immunofluorescence staining and western blot and is capable of detecting endogenous protein. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band at ~ 120 kDa corresponding to full-length protein by western blotting in the appropriate cell lysate or extract. Higher MW bands may be seen that may be due to hyper-phosphorylation of the protein. The use of HeLa whole cell lysates is recommended as a positive control. For IF microscopy use cells grown on cover slips fixed with 3.5% paraformaldehyde in PBS at pH 6.8. Permeabilize fixed cells with 0.2% Triton X-100 in 25 mM Tris Cl, pH 7.4 containing 0.1% BSA.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
BUBR1 Kinase (BUB1-related protein kinase) is a probable component of the mitotic checkpoint that delays anaphase until all chromosomes are properly attached to the mitotic spindle. This protein can interact with BUB3, CENP-F, CENP-E and mitosin. BUBR1 Kinase is found within the cytoplasm in interphase cells, but when bound to BUB3 or CENP-E, it can be localized to nuclear kinetochores. This protein is highly expressed in thymus followed by spleen. Defects in BUB1B are associated with tumor formation.
Lázaro-Diéguez et al. (2015). Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum. Molecular Biology of the Cell
Applications
IF, Confocal Microscopy; Multiplex Assay
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.