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BECLIN2 Antibody
Rabbit Polyclonal
600-401-Z52
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC, IF
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Beclin2 (RABBIT) Antibody - 600-401-Z52
BECN2, BECN1L1, Beclin-1-like protein 1, Beclin 1 autophagy related pseudogene 1, BECN1P1, BECLIN 2, BECLIN-2
Rabbit
Polyclonal
IgG
Target Details
BECN2 - View All BECN2 Products
Human
Conjugated Peptide
Anti-Beclin 2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a 16 amino acid peptide near the N-terminus of human Beclin 2.
Anti-Beclin 2 antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography. Beclin 2 antibody is human specific. At least two isoforms of Beclin 2 are known to exist; this antibody will only detect the longer isoform. Beclin 2 antibody is predicted to not cross-react with Beclin 1.
Application Details
ELISA, IF, IHC, WB
Anti-Beclin 2 Antibody has been tested for use in ELISA, immunohistochemistry, immunofluorescence, and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 48 kDa in Western Blots of specific cell lysates and tissues.
Formulation
1.0 mg/ml by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells (1,2). Beclin 2 is a mammalian specific homolog of the autophagy protein Beclin 1 (3). Like Beclin 1, Beclin 2 interacts with Bcl-2 and class III PI3K complex components. However, Beclin 2 functions in an additional lysosomal degradation pathway and is required for ligand-induced endolysosomal degradation of several G protein-coupled receptors (3). Beclin 2 is also required for agonist-induced lysosome-mediated degradation of EGFR in lung cancer cells, suggesting that it may also play a role in regulating other intracellular signaling pathways (4).
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.