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B-raf Antibody
Chicken Polyclonal
200-901-Z86
100 µg
Liquid (sterile filtered)
WB, ELISA
Human, Mouse, Rat
Chicken
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-B-raf (CHICKEN) Antibody - 200-901-Z86
B-raf Antibody, NS7, BRAF1, RAFB1, B-RAF1, Proto-oncogene B-Raf
Chicken
Polyclonal
IgY
Target Details
BRAF - View All BRAF Products
Human, Mouse, Rat
Conjugated Peptide
Anti-B-raf antibody was prepared from chicken egg fractions produced by repeated immunizations with an 18 amino acid synthetic peptide near the internal region of human B-raf. The immunogen is located within amino acids 330 - 380 of B-raf.
Anti-B-raf Antibody is an IgY fraction that has been affinity purified by immunoaffinity purification. Cross reactivity with B-raf from other sources has not been determined.
Application Details
ELISA, WB
Anti-B-raf Antibody has been tested for use in ELISA and Western Blotting. Specific conditions for reactivity should be optimized by the end user. Expect a band at approximately 94 kDa in Western Blots of specific cell lysates and tissues. Positive controls used are human brain tissue, HEK293 Cells, Daudi Cells, and K562 Cells.
Formulation
1 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
B-raf belongs to the raf/mil family of serine/threonine protein kinases and plays a role in regulating the MAP kinase/ERKs signaling pathway, which affects cell division, differentiation, and secretion. The Ras/Raf/MEK/ERK and Ras/PI3K/PTEN/Akt pathways interact with each other to regulate growth and in some cases tumorigenesis. Mutations in B-raf have been associated with several cancers, including non-Hodgkin lymphoma, colorectal cancer, malignant melanoma, thyroid carcinoma, non-small cell lung carcinoma, and adenocarcinoma of lung, leading to speculation on the possibility of B-raf as a therapeutic target for treating cancers. Mutations in this gene have also been associated with cardiofaciocutaneous syndrome (CFCS), a disease characterized by heart defects, mental retardation and a distinctive facial appearance.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.