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B-Phycoerythrin Antibody
Rabbit Polyclonal
6 References
200-4199
200-401-099S
200-401-099
50 mg
25 µL
100 µg
Lyophilized
Liquid (sterile filtered)
Liquid (sterile filtered)
ELISA, IHC, IF, Multiplex, Other
Phycoerythrin
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-B-PHYCOERYTHRIN (RABBIT) Antibody (BULK ORDER) - 200-4199
rabbit anti-B-Phycoerythrin Antibody, rabbit anti B-Phycoerythrin, rabbit anti Phycoerythrin
Rabbit
Polyclonal
IgG
Target Details
cpeA, cpeB
Phycoerythrin
Native Protein
Phycoerythrin [Porphyridium creuentum]
Anti-B-Phycoerythrin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phycoerythrin [Porphyridium creuentum].
Application Details
ELISA
IF, IHC, Other, Multiplex
- View References
Anti-B-Phycoerythrin antibody has been tested by ELISA and is suitable for western blot, immunohistochemistry, immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
5.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-B-Phycoerythrin Antibody detects the B-PE phycoerythrin protein. Phycoerythrin is a red protein from the light-harvesting phycobiliprotein family, present in cyanobacteria, and red algae. Phycoerythrin is organized in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. R-Phycoerythrin and B-Phycoerythrin are among the brightest biological fluorescent dyes and are commonly used as a fluorescent dye in many assays and is frequently conjugated to antibodies as a reporter molecule. The spectral properties of B-Phycoerythrin are absorption at 545 nm and emission at 572 nm.
Pirgova G et al. (2020). Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen. Proc Natl Acd Sci USA.
Applications
Immunization
Kerry Tedford et al. (2017). The opposing forces of shear flow and sphingosine-1-phosphate control marginal zone B cell shuttling. Nat Commun.
Applications
IHC, ICC, Histology
Rodda LB et al. (2015). Phenotypic and Morphological Properties of Germinal Center Dark Zone Cxcl12-Expressing Reticular Cells. J Immunol.
Applications
CLARITY, Two-Photon Microscopy
Wang W et al. (2014). Long-term B cell depletion in murine lupus eliminates autoantibody-secreting cells and is associated with alterations in the kidney plasma cell niche. J Immunol.
Applications
IF, Confocal Microscopy; Multiplex Assay
Green JA et al. (2011). The sphingosine 1-phosphate receptor S1P₂ maintains the homeostasis of germinal center B cells and promotes niche confinement. Nat Immunol.
Applications
CLARITY, Two-Photon Microscopy
de Vries IJM et al. (2007). In situ detection of antigen-specific T cells in cryo-sections using MHC class I tetramers after dendritic cell vaccination of melanoma patients. Cancer Immunol Immunother.
Applications
IHC, ICC, Histology
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.