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Avi Epitope Tag Antibody
Rabbit Polyclonal
1 References
100-401-B21
100 µL
Liquid (sterile filtered)
WB, ELISA
Avi-Tag
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Avi Epitope Tag (RABBIT) Antibody - 100-401-B21
rabbit anti-Biotin Ligase Epitope Tag Antibody, rabbit anti-Avi-tag antibody, biotin ligase, biotin operon repressor protein, biotin holoenzyme synthetase, biotin-[acetyl-CoA carboxylase] synthetase
Rabbit
Polyclonal
Antiserum
Target Details
Avi-Tag
Conjugated Peptide
Anti-Avi Epitope Tag antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic avi-tag peptide GLNDIFEAQKIEWH (minimal BirA substrate; biotin is added to the sequence at the lysine).
This antiserum detects the minimal peptide substrate GLNDIFEAQKIEWH for the E. coli biotin ligase BirA. Western blot analysis of B. subtilis strains producing either (Avi-tagged) SPoIIIAH (XWB49) or (Avi-tagged) SpoIIQ (XWB46) showed strong and specific reactivity to the antisera.
Application Details
ELISA, WB
Anti-Avi Epitope Tag Antibody has been tested in ELISA and Western blot. Minor background bands of unknown identity may be observed. The antisera will recognize the Avi-tag irrespective of the whether the tag is biotinylated (data not shown).
Formulation
90 mg/ml by Refractometry
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The peptide is also called the biotin acceptor peptide (BAP) of the biotin carboxyl carrier protein (BCCP) subunit of acetyl CoA carboxylase of E. coli.
Abelin JG et al. (2019). Defining HLA-II ligand processing and binding rules with mass spectrometry enhances cancer epitope prediction. Immunity.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.