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ATF4 Antibody Peroxidase
Mouse Monoclonal S360A-24 IgG2a
200-303-W61
100 µg
Liquid (sterile filtered)
WB, IHC
Human, Rat
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-ATF4 (MOUSE) Monoclonal Antibody Peroxidase Conjugated - 200-303-W61
Activating Transcription Factor 4, ATF 4, ATF4 protein, cAMP-dependent transcription factor ATF-4, cAMP-responsive element-binding protein 2, CREB 2, CREB-2, CREB2, Cyclic AMP dependent transcription factor ATF 4
Mouse
Peroxidase (HRP)
Monoclonal
IgG2a
Target Details
ATF4 - View All ATF4 Products
Human, Rat
Recombinant Protein
Anti-ATF4 Antibody was produced by repeated immunization of mice with a fusion protein containing amino acids 25-327 of human ATF4.
Anti-ATF4 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography. BLAST analysis suggests that it is 85% identical to mouse, 86% identical to rat and <50% identical to ATF5. Will not cross react with ATF5.
Application Details
IHC, WB
Anti-ATF4 HRP Conjugated Antibody is tested for Western Blots and Immunocytochemistry. Expect a band approximately ~60 kDa on specific lysates or tissues. Does not cross react with ATF5. Specific conditions for reactivity should be optimized by the end user.
Formulation
1mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1% (w/v) Sodium Azide
50% (v/v) Glycerol
Shipping & Handling
Wet Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Cyclic AMP-dependent transcription factor ATF-4 (ATF4) is a basic leucine-zipper (bZip) transcription factor, which regulates amino acid metabolism, DNA damage repair, chromatin remodeling, and apoptosis in response to cellular and ER stress. ATF4 works with various proteins, such as C/EBP homology protein (CHOP), aspargine synthetase (ASNS), and cAMP response element (CRE) among others to mediate cellular stress. ATF4 also regulates glucose homeostasis by suppressing beta-cell proliferation and insulin production. Furthermore, ATF4 targets the histone demethylase JMJD3 to alter chromatin structure and enhance gene transcription in response to amino acid deprivation. Anti-ATF4 is ideal for researchers interested in Cell Signaling, Oncology, Cell Differentiation, and Apoptosis; relevant pathways include MAPK signaling pathways, Activation of cAMP-Dependent PKA, CREB pathways, GPCR pathways and Rho Family GTPases.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.