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Aquaporin 2 phospho S269 Antibody
Rabbit Polyclonal
612-401-D10
100 µL
Liquid
WB, IHC, IP
Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Aquaporin 2 pS269 (RABBIT) Antibody - 612-401-D10
ADH water channel, Aquaporin-CD, Collecting duct water channel protein, WCH-CD, Water channel protein for renal collecting duct
Rabbit
Polyclonal
IgG
Target Details
Aqp2 - View All Aqp2 Products
Rat
Phosphorylation
Conjugated Peptide
Anti-Aquaporin 2 pS269 Antibody was produced by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Ser269.
Anti-Aquaporin 2 pS269 Antibody is directed against rat Aquaporin 2 proteins phosphorylated at S269. The antibody was prepared from monospecific antiserum by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide and non-specific peptide to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum. Minimal reactivity occurs against non-phosphorylated Aquaporin 2. Reactivity against Aquaporin 2 occurs from rat sources. However, reactivity is also expected against the following species based on 100% sequence homology: bovine, canine and mouse.
P34080 - UniProtKB
Application Details
IHC, IP, WB
Anti-Aquaporin 2 pS269 antibody is tested for use in ICC, IHC, IP, and WB. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 29 kDa in size corresponding to AQP2 protein phosphorylated at Ser269 in the appropriate cell lysate or extract. This antibody also recognizes the glycosylated form of aquaporin 2 protein at approximately 37 kDa in size.
Formulation
Titrated value sufficient to run approximately 10 mini blots.
0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5
0.1 mg/ml Bovine Serum Albumin (BSA) - IgG and Protease free, 50% (v/v) Glycerol
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Aquaporin 2 antibody recognizes aquaporin 2 (AQP2) which is a hormonally regulated water channel located in the renal collecting duct. Mutations in the AQP2 gene cause hereditary nephrogenic diabetes insipidus in humans. A vasopressin induced cAMP increase results in the phosphorylation of AQP2 at serine-256 and its translocation from the intracellular vesicles to the apical membrane of principal cells. Serine-269 has been recently identified as a vasopressin-mediated phosphorylation site on AQP2 and as such has shown to potentiate plasma membrane retention of AQP2. Anti-Aquaporin 2 pS269 antibody is ideal for investigators involved in Cell Signaling, Neuroscience, Signal Transduction research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
