APOLIPOPROTEIN J Antibody
Goat Polyclonal
3 References
600-101-198
500 µg
Liquid (sterile filtered)
WB
Human
Goat
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Apolipoprotein J (GOAT) Antibody - 600-101-198
goat anti-Apolipoprotein J antibody, Clusterin, Complement-associated protein SP-40, Complement cytolysis inhibitor, CLI, NA1/NA2, Apo-J, Testosterone-repressed prostate message 2, TRPM-2, Ku70-binding protein 1, Aging-associated gene 4 protein, Clusterin beta chain, Clusterin alpha chain, Complement cytolysis inhibitor a chain, Complement cytolysis inhibitor b chain, ApoJ alpha, ApoJ beta, CLU, CLI, KUB1, AAG4
Goat
Polyclonal
IgG
Target Details
CLU - View All CLU Products
Human
Native Protein
apoLipoprotein Type J was isolated from human plasma by density gradient centrifugation followed by HPLC purification.
This product has been prepared by immunoaffinity chromatography using immobilized antigens followed by extensive cross-adsorption against other apoLipoproteins and human serum proteins to remove any unwanted specificities. Typically less than 1% cross reactivity against other types of apoLipoprotein was detected by ELISA against purified standards. This antibody reacts with human apoLipoprotein J and has negligible cross-reactivity with Type A-I, A-II, B, C-I, C-II, C-III and E apoLipoproteins. Specific cross reaction of anti-apoLipoprotein antibodies with antigens from other species has not been determined. Non-specific cross reaction of anti-apoLipoprotein antibodies with other human serum proteins is negligible.
Application Details
WB
- View References
Anti-apolipoprotein antibodies have been used for indirect trapping ELISA for quantitation of antigen in serum using a standard curve, for immunoprecipitation and for western blotting for highly sensitive qualitative analysis.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.125 M Sodium Borate, 0.075 M Sodium Chloride, 0.005 M EDTA, pH 8.0
0.01% (w/v) Sodium Azide
None
Shipping & Handling
Wet Ice
Store vial at 4° C prior to opening. This product is stable at 4° C as an undiluted liquid. Dilute only prior to immediate use. For extended storage mix with an equal volume of glycerol, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing.
Expiration date is one (1) year from date of receipt.
Apolipoprotein J Antibody functions as a secreted chaperone that prevents aggregation of nonnative proteins. It prevents stress-induced aggregation of blood plasma proteins and inhibits the formations of amyloid fibrils. Apolipoprotein J does not require ATP or refold proteins by itself. It maintains partially unfolded proteins in a state for subsequent refolding by other chaperones. It is shown to be involved in several basic biological events such as cell death, tumor progression, and neurodegenerative disorders. Binding to cell surface receptors it triggers internalization of chaperone-client complex and subsequent lysosomal or proteasomal degradation. It modulates NF-kappa-B transcriptional activity. Nuclear isoforms promote apoptosis while mitochondrial isoforms suppress BAX-dependent release of cytochrome c into the cytoplasm and inhibit apoptosis. Anti-Apolipoprotein J Antibody is useful for researchers interested in the immune system, Ubiquitin pathways, and cardiovascular research.
Sepulveda J et al. (2022). Independent APOE4 knock-in mouse models display reduced brain APOE protein, altered neuroinflammation, and simplification of dendritic spines. J Neurochem.
Applications
WB, IB, PCA
Hayashi H et al. (2021). Apolipoprotein E-Containing Lipoproteins and LRP1 Protect From NMDA-Induced Excitotoxicity Associated With Reducing α2-Macroglobulin in Müller Glia. Invest Ophthamol Vis Sci.
Applications
WB, IB, PCA
Hayashi H et al. (2009). Protection of neurons from apoptosis by apolipoprotein E-containing lipoproteins does not require lipoprotein uptake and involves activation of phospholipase Cgamma1 and inhibition of calcineurin. J Biol Chem.
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
