Datasheet

APC1 Antibody

Name: APC1 Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

Item No. 600-401-857

Size: 100 µg

Physical State: Liquid (sterile filtered)

Applications: ELISA

Reactivity: Human

Host Species: Rabbit

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Specifications for APC1 Antibody

Product Details

Description: Anti-APC1 S355 pan reactive (RABBIT) Antibody - 600-401-857

Synonyms: rabbit anti-APC1 Antibody, APC-1, APC 1, rabbit anti-APC1 S355 Antibody, Anaphase-promoting complex subunit 1, cyclosome subunit 1, Testis-specific gene 24 protein, Mitotic checkpoint regulator

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: ANAPC1, TSG24 - View All ANAPC1, TSG24 Products

Reactivity: Human

Immunogen Type: Conjugated Peptide

Immunogen: This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acids 350-375 of Human Apc 1. Swiss Prot accession number Q9H1A4.

Purity/Specificity: This affinity purified antibody is directed against human APC1at the S355 residue. The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Database Links:

Q9H1A4 - UniProtKB

12056971 - NCBI Protein

64682 - Gene ID

Application Details

Tested Applications: ELISA

Application Note: This affinity purified antibody has been tested for use in ELISA against the immunizing peptide. Reactivity in other immunoassays is unknown.

Formulation

Concentration: 1.2 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

Background

APC1 (also known as Anaphase promoting complex subunit 1, Cyclosome subunit 1, Protein Tsg24, Mitotic checkpoint regulator and ANAPC1) is 1 of at least 11 subunits of the anaphase-promoting complex (APC), which functions at the metaphase-to-anaphase transition of the cell cycle and is regulated by spindle checkpoint proteins. The APC is an E3 ubiquitin ligase that targets cell cycle regulatory proteins for degradation by the proteasome, thereby allowing progression through the cell cycle.

Related Protocols

ELISA Protocol

Sandwich ELISA Protocol for Collagen

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.