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APAF1 Antibody [2E10]
Mouse Monoclonal 2E10 IgG
200-301-A35
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC
Human, Mouse, Rat
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-APAF1 (MOUSE) Monoclonal Antibody - 200-301-A35
Apoptotic peptidase activating factor 1 antibody, Apoptotic protease activating factor 1 antibody, Apoptotic protease activating factor antibody, CED 4 antibody, CED4 antibody, KIAA0413 antibody, Apaf1, Apaf-1
Mouse
Monoclonal
IgG
Target Details
APAF1 - View All APAF1 Products
Human, Mouse, Rat
Conjugated Peptide
Anti-Apaf1 Antibody monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with a synthetic peptide corresponding to a region near the carboxy terminus of human Apaf1 protein.
Apaf1 Antibody is directed against human Apaf1 protein. The product was purified from Ascites by immunoaffinity chromatography purified IgG. Expect reactivity with human, mouse and rat Apaf1. Cross-reactivity with Apaf1 from other sources has not been determined.
Application Details
ELISA, IHC, WB
Anti-Apaf1 Antibody has been tested for use in ELISA, immunohistochemistry and western blotting. Specific conditions for reactivity should be optimized by the end user. Paraformaldehyde fixation is recommended for immunohistochemical staining. Expect a band at ~130 kDa in size corresponding to Apaf1 by western blotting in the appropriate cell lysate or extract. K562 cells can be used as a positive control.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Apoptosis is related to many diseases and is induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologue of the key cell death gene CED-4 in C. elegans was identified recently from human and mouse and designated Apaf1 for apoptosis protease-activating factor 1. Apaf1 binds to cytochrome c (Apaf2) and caspase-9 (Apaf3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3, one of the key proteases responsible for the proteolytic cleavage of many key proteins in apoptosis. Apaf1 can also associate with caspase-4 and caspase-8. Apaf1 transcript is ubiquitously expressed in human tissues. Anti-Apaf1 antibody is ideal for investigators that are involved in Apoptosis, Cytokine and growth factor research.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
