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AML1-ETO Antibody
Rabbit Polyclonal IgG
1 References
100-401-V33
100 µL
Liquid (sterile filtered)
WB, ELISA, ChIP, Other
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-AML1-ETO (RABBBIT) Antibody - 100-401-V33
Runt-related transcription factor 1, Acute myeloid leukemia 1 protein, Core-binding factor subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus enhancer-binding protein 2 alpha B subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 enhancer factor 1 alpha B subunit, SL3/AKV core-binding factor alpha B subunit
Rabbit
Polyclonal
Antiserum
Target Details
RUNX1 - View All RUNX1 Products
Human
Conjugated Peptide
Anti-AML1-ETO Antibody was produced in rabbits by repeated immunizations with a synthetic peptide of human AML1-ETO.
Anti-AML1-ETO Antibody is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This product reacts with human AML1-ETO. Cross reactivity with AML1-ETO from other sources is not known.
Application Details
ChIP, ELISA, WB
Other
- View References
Anti-AML1-ETO Antibody has been tested in ChIP, ELISA and Western Blots. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 80 kDa in the appropriate cell lysate or extract.
Formulation
neat
None
0.05% (w/v) Sodium Azide and 0.05% ProClin 300
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
This antibody specifically recognizes the AML1 (RUNX1) (UniProtKB/Swiss-Prot entry Q01196) - ETO (RUNX1T1) (UniProtKB/Swiss-Prot entry Q06455) fusion protein that arises due to a translocation between chromosome 8 and 22. This translocation is one of the most frequent karyotypic abnormalities observed in acute myeloid leukemia. It produces a chimerical gene made up of the 5’-region of AML1and the 3’-region of ETO. The chimerical protein is thought to associate with the nuclear corepressor/histone deacetylase complex to block hematopoietic differentiation. Anti-AML1-ETO Antibody is ideal for research in Cancer and Gene Expression.
Qi X et al. (2021). HDN-1 induces cell differentiation toward apoptosis in promyelocytic leukemia cells depending on its selective effect on client proteins of Hsp90. Toxicol Appl Pharmacol.
Applications
Undefined
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
