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Alpha A Crystallin Antibody
Mouse Monoclonal 1H3.B8 IgG1
1 References
200-301-E84
200 µg
Liquid (sterile filtered)
WB, IF
Human, Mouse, Rat, Bovine
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Alpha A Crystallin (MOUSE) Monoclonal Antibody - 200-301-E84
Heat shock protein beta4, Acry 1, CRYA1, CRYAA, HspB4, Alpha-crystallin A chain
Mouse
Monoclonal
IgG1
Target Details
CRYAA - View All CRYAA Products
Human, Mouse, Rat, Bovine
Native Protein
Alpha Crystallin Antibody was produced in mice by repeated immunizations with Native Alpha Crystallin protein.
Anti-Alpha A Crystallin Antibody was purified by Protein G chromatography. A BLAST analysis was used to suggest cross-reactivity with Alpha A Crystallin from Human, Mouse, Cow, and Rat based on 100% homology with the immunizing sequence. Does not cross-react with αB-crystallin, βL-crystallin, ΒH- crystallin, γ-crystallin, Hsp25, Hsp27 or Hsp47 proteins. Cross-reactivity with Alpha A Crystallin from other sources has not been determined. Chaperones & Trafficking research.
P02489 - UniProtKB
NP_000385.1 - NCBI Protein
Application Details
IF, WB
Anti-Alpha A Crystallin Antibody has been tested in WB and IF and is recommended for use in ELISA. Expect a band approximately ~20kDa protein corresponding to the molecular mass of α-crystallin on SDS PAGE immunoblots. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.0 mg/ml by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
50% (v/v) Glycerol
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The alpha-crystallins are major water-soluble lens structural proteins of the vertebrate eye that are related to the small heat shock protein family. The alpha-crystallins possess structural and functional similarities with Hsp25 and Hsp27. Mammalian lens cystallins are divided into alpha, beta and gamma families. Alpha and beta families are further divided into acidic and basic groups (Alpha-A and Alpha-B respectively). In the lens, alpha-crystallin primarily functions to maintain proper refractive index, however it can also function as a molecular chaperone that binds to the denatured proteins, keeping them in solution and thereby maintaining the translucency of the lens. When cellular stress occurs, alpha-crystallin enters its’ phosphorylated state and may serve a structural control function and play a role in protein maintenance. In addition to their interaction with proteins, alpha-crystallins also interact with native molecules such as membrane proteins, Golgi matrix protein, structural proteins, nuclear proteins and DNA. Two other functions are an autokinase activity and participation in the intracellular architecture, and it has also been proven that both alpha-A and B prevent apoptosis by inhibiting caspases.
Wei Z et al. (2023). A tamoxifen-inducible Cre knock-in mouse for lens-specific gene manipulation. Exp Eye Res.
Applications
Undefined
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.