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ALKALINE PHOSPHATASE Antibody Peroxidase Conjugated
Rabbit Polyclonal
200-403-142
200-403-142S
200-403-142-0100
20 mg
25 µL
100 µg
Lyophilized
Liquid (sterile filtered)
Lyophilized
Human
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Alkaline Phosphatase (Human Intestine) (RABBIT) Antibody Peroxidase Conjugated (BULK ORDER) - 200-403-142
rabbit anti-Alkaline Phosphatase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Alkaline phosphatase intestinal antibody, Alkaline phosphomonoesterase antibody, ALPI antibody, Glycerophosphatase antibody, IAP antibody, Intestinal alkaline phosphatase antibody, Kasahara isozyme antibody
Rabbit
Peroxidase (HRP)
Polyclonal
IgG
Target Details
ALP1 - View All ALP1 Products
Human
Native Protein
Alkaline Phosphatase [Human Intestine]
Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [Human Intestine]. Cross reactivity against Alkaline Phosphatase from other sources may occur but has not been specifically determined.
Application Details
Anti-Alkaline Phosphatase has been assayed against 1.0 ug of Alkaline Phosphatase [Human Intestine] by immunoblot using TMB 3,3',5,5'Tetramethylbenzidine code # TMBM-100 as a substrate at room temperature. A working dilution of 1:1,000 to 1:5,000 of the reconstitution concentration is suggested for this product.
Formulation
10 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Alkaline phosphatase (ALP, ALKP) is a hydrolase enzyme responsible for removing phosphate groups from many types of molecules, including nucleotides, proteins, and alkaloids. The process of removing the phosphate group is called dephosphorylation. All mammalian alkaline phosphatase isoenzymes except placental (PALP and SEAP) are inhibited by homoarginine, and, in similar manner, all except the intestinal and placental ones are blocked by levamisole. Heating for ~2 hours at 65°C inactivated most isoenzymes except Placental isoforms (PALP and SEAP).
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
