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Alkaline Phosphatase Antibody
Rabbit Polyclonal
1 References
200-4134
200-4134S
200-4134-0100
50 mg
25 µL
100 µg
Lyophilized
Liquid (sterile filtered)
Lyophilized
WB, IF
E. coli
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Alkaline Phosphatase (E. coli) (RABBIT) Antibody (BULK ORDER) - 200-4134
rabbit anti-Alkaline Phosphatase Antibody, Akp2 antibody, ALP antibody, Nagao isozyme antibody, Placental alkaline phosphatase antibody, Placental like alkaline phosphatase antibody, PLAP 1 antibody, PLAP like antibody, Regan isozyme antibody, Kasahara isozyme antibody
Rabbit
Polyclonal
IgG
Target Details
phoA - View All phoA Products
E. coli
Native Protein
Alkaline Phosphatase [E.coli]
Anti-Alkaline Phosphatase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Alkaline Phosphatase [E.coli]. Cross reactivity against Alkaline Phosphatase from other tissues and species may occur but have not been specifically determined. Some minimal cross reactivity has been reported when lysates of cell extracts overproducing E.coli proteins are probed.
Application Details
WB
IF
- View References
Anti-Alkaline Phosphatase Antibody has been tested against 1.0 ug of Alkaline Phosphatase [E.coli] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) code #611-1302 and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature. A working dilution of 1:25,000 to 1:125,000 of the reconstitution concentration is suggested for this product. A 1:10,000 dilution has been reported to be useful for the detection by immunoblot of an integral membrane protein from a gram-positive organism expressed as a LacZ/PhoA chimeric derivative in E.coli. The researcher should determine optimal titers for other applications.
Formulation
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
5.0 mL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Sobajima, T et al. (2014). Rab11a is required for apical protein localisation in the intestine. Biology Open
Applications
WB, IB, PCA; IF, Confocal Microscopy
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
