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Datasheet

Alanine Transaminase Antibody Biotin Conjugated

Sheep Polyclonal

200-606-206 200-606-206S 200-606-206-0100
10 mg 25 µL 100 µg
Lyophilized Liquid (sterile filtered) Lyophilized
Pig
Sheep
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$125.00 /Per Item
$455.00 /Per Item
10 mg $0.00
25 µL $125.00
100 µg $455.00
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Product Details

Anti-Alanine Transaminase (SHEEP) Antibody Biotin Conjugated (BULK ORDER) - 200-606-206
sheep anti-Alanine Transaminase Antibody, biotin conjugated sheep anti-Alanine Transaminase Antibody, AAT1 antibody, Alanine aminotransferase 1 antibody, alanine aminotransferase antibody, ALT 1 antibody, ALT1 antibody, Glutamic alanine transaminase 1 antibody
Sheep
Biotin
Polyclonal
IgG

Target Details

GPT  - View All GPT Products
Pig
Native Protein
Alanine Transaminase (ALT) [Pig Heart]
Alanine Transaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Sheep Serum as well as purified and partially purified Alanine Transaminase (ALT) [Pig Heart].  Cross reactivity against Alanine Transaminase (ALT) from other sources is unknown.
P13191 - UniProtKB
113565 - NCBI Protein
2875 - Gene ID

Application Details

Anti-Alanine Transaminase has been assayed against 1.0 ug of Alanine Transaminase (ALT) in a standard capture ELISA using Peroxidase Conjugated Streptavidin #S000-03 and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:4,000 to 1:20,000 of the reconstitution concentration is suggested for this product.

Formulation

10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Anti-Alanine Transaminase antibody is part of the class-I pyridoxal-phosphate-dependent aminotransferase family. It catalyzes the transfer of the alanine amino group, reversible transamination, to produce pyruvate and glutamate. Located in the cytoplasm, this antibody is a factor in cellular nitrogen metabolism and liver gluconeogenesis. Anti-Alanine Transaminase antibody is ideal for investigators interested in Metabolism, Signal Transduction, and Cancer research.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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