AKT1 Western Chemiluminescent Blotting Kit

Western Blot of Mouse Anti-AKT1 Antibody

PAGE-MAP (microsphere affinity proteomics) of Mouse Anti-AKT1 Antibody

Monoclonal mouse anti AKT1 - WB - Cell lines

Datasheet Protocol

AKT1 Western Chemiluminescent Blotting Kit

Name: AKT1 Western Chemiluminescent Blotting Kit
Brand: Rockland Immunochemicals
Price: 385.0 USD
Availability: OutOfStock

Mouse Monoclonal 14E5.A2.B2.H9 IgG2a kappa

Item No. KCAI51

Size: 1 Kit

Physical State: n/a

Applications: WB, ELISA, IF

Reactivity: Human

Host Species: Mouse

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Specifications for AKT1 Western Chemiluminescent Blotting Kit

Product Details

Description: AKT1 Western Blot Kit - KCAI51

Synonyms: AKT 1 western blotting, AKT-1, PKB antibody, PKB gamma antibody, PKBGAMMA antibody, PRKBG antibody, Protein kinase Akt 1 antibody, Protein kinase B gamma antibody, RAC-gamma serine/threonine-protein kinase, RAC-PK-gamma, Chemiluminescent WB, Western Blotting Kit, Chemiluminescent Kit, Peroxidase Kit, Immunoblotting Kit

Host Species: Mouse

Clonality: Monoclonal

Format: IgG2a

Detection Kit Type: Chemiluminescent Western Blot Kit

Target Details

Gene Name: AKT1 - View All AKT1 Products

Reactivity: Human

Purity/Specificity: The kit is designed to detect both unphosphorylated and phosphorylated forms of the protein. Cross reactivity with AKT1 from other species has not been determined, however, the sequence of the immunogen shows 100% identity to human, mouse, and rat, therefore, cross reactivity is expected. Cross-reactivity with AKT2 and AKT3 has not been determined. This kit contains sufficient substrate for up to 5 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.

Database Links:

P31749 - UniProtKB

Application Details

Tested Applications: ELISA, IF, WB

Application Note: Use Rockland Immunochemicals’ Anti-AKT1 Chemiluminescent Kit for Western Blotting for detection of human AKT1 native and recombinant proteins by western blot. Expect a band approximately 56 kDa in size corresponding to AKT1 protein. This kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.

Formulation

Preservative: Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: See kit insert for complete instructions.

Expiration: See kit insert for complete instructions.

Background

Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for AKT1 combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of native and/or recombinant human AKT1 protein. AKT1 Western Blotting kit allows for the detection of endogenous protein levels of human AKT1 present in cell lysates provided by the user. As a positive control, this kit includes a MDA-MB468 whole cell lysate proven to contain AKT1. After protein separation by SDS-PAGE and transfer, the membrane is probed with Rockland's optimized Anti-AKT1 antibody. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems. AKT1 Western Blotting is ideal for investigators involved in Cell Signaling, Cancer, Neuroscience and Signal Transduction research.

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.