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AIF Antibody
Rabbit Polyclonal
2 References
200-401-985
100 µg
Liquid (sterile filtered)
WB, ELISA, IHC
Human, Mouse, Rat
Rabbit
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-Apoptosis Inducing Factor (AIF) (RABBIT) Antibody - 200-401-985
Harlequin antibody, Hq antibody, mAIF antibody, MGC111425 antibody, MGC5706 antibody, PDCD 8 antibody, AIF, PDCD8
Rabbit
Polyclonal
IgG
Target Details
AIFM1 - View All AIFM1 Products
Human, Mouse, Rat
Conjugated Peptide
AIF Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids at an internal region of human AIF protein.
Anti-AIF Antibody is directed against human AIF protein. The product is affinity chromatography purified via peptide column. A BLAST analysis was used to suggest cross-reactivity with AIF protein from mouse and rat based on 100% homology with the immunizing sequence. Reactivity against homologues from other sources is not known.
Application Details
ELISA, IHC, WB
Anti-AIF Antibody has been tested for use in ELISA, western blotting and immunohistochemistry. K562 cell lysate, as well as rat and mouse heart tissue lysates, can be used as positive controls in western blotting, and a band at approximately 67 kDa is expected. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.02% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of the caspase family, caspase activated DNase, and several novel proteins. A novel gene, the product of which causes chromatin condensation and DNA fragmentation, was recently identified, cloned, and designated apoptosis inducing factor (AIF). Like cytochrome c and caspase-9, which are critical molecules in apoptosis, AIF localizes to mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis. These effects occur in both isolated nuclei and in the nuclei of live cells treated by microinjection and with apoptosis stimuli. AIF is highly conserved between human and mouse and is widely expressed. Anti-AIF antibody is ideal for investigators involved in DNA Damage and Repair and Chromatin research.
Wang Z et al. (2019). Decreased expression of apoptosis‑inducing factor in renal cell carcinoma is associated with poor prognosis and reduced postoperative survival. Oncol Lett.
Applications
IHC, ICC, Histology
Koh et al. (2015). Enhanced cytotoxicity in triple-negative and estrogen receptor‑positive breast adenocarcinoma cells due to inhibition of the transient receptor potential melastatin-2 channel. Oncology Reports
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.