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8-Hydroxy Guanine Antibody
Mouse Monoclonal 15A3 IgG2b
5 References
200-301-A99
100 µg
Liquid (sterile filtered)
WB, IHC, IF, IP
Human, Mouse, Rat
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-8-Hydroxy Guanine (MOUSE) Monoclonal Antibody - 200-301-A99
8 hydroxy 2’ deoxyguanosine antibody, 8 hydroxyguanine antibody, 8 hydroxyguanosine antibody, 8 OHG antibody, 8-OHG antibody, 8OG antibody, 8OHdG antibody, 8OHG antibody, 8-Hydroxy Guanine Antibody, 8-OH-dG Antibody, DNA/RNA Damage Antibody
Mouse
Monoclonal
IgG2b
Target Details
Human, Mouse, Rat
Native Protein
This Protein G purified monoclonal antibody was prepared using conventional hybridoma technology after repeated immunizations with 8-hydroxy-guanosine-BSA and casein conjugates.
This Protein G purified Anti-8-Hydroxy Guanine monoclonal antibody recognizes markers of oxidative damage to DNA (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine).
Application Details
IHC
IF, IP, WB
- View References
This Protein G purified antibody has been tested for use in immunohistochemistry, ICC/IF, Dot Blot, IP, Flow Cytometry, and ELISA. Specific conditions for reactivity should be optimized by the end user.
Formulation
1.0 mg/mL by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1% (w/v) Sodium Azide
50% (v/v) Glycerol
Shipping & Handling
Dry Ice
Store Anti-8-Hydroxy Guanine antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
DNA or RNA damage is due to environmental factors and normal metabolic processes inside the cell, that then hinder the ability of the cell to carry out its functions. There are four main types of DNA damage due to endogenous cellular processes: oxidation, alkylation, hydrolysis and mismatch of the bases. During the oxidation of bases, highly reactive chemical entities collectively known as RONS may develop. RONS stands for reactive oxygen and nitrogen species and includes nitric oxide, superoxide, hydroxyl radical, hydrogen peroxide and peroxynitrite. Numerous studies have shown that RONS cause a variety of other issues in addition to DNA damage. 8-hydroxyguanine, 8-hydroxy-2’-deoxyguanosine and 8-hydroxyguanosine are all RNA and DNA markers of oxidative damage. 8-hydroxy-2’-guanosine is produced by reactive oxygen and nitrogen species including hydroxyl radical and peroxynitrite. Specifically its high biological relevance is due to its ability to induce G to T transversions, which is one of the most frequent somatic mutations (2). 8-hydroxy-guanine has been the most frequently studied type of DNA base damage, with studies in diabetes, and cancer. Base modifications of this type arise from radical-induced hydroxylation and cleavage reactions of the purine ring. Finally, 8-hydroxy-guanosine, like 8-hydroxy-2’-guanosine, induces a mutagenic transversion of G to T in DNA. Its role has been tested specifically in the development of diabetes, hypertension and strokes.
Li R et al. (2024). Alzheimer's Amyloid-β Accelerates Cell Senescence and Suppresses SIRT1 in Human Neural Stem Cells. Biomolecules.
Applications
IF, Confocal Microscopy
Peng J et al. (2020). Idebenone attenuates cerebral inflammatory injury in ischemia and reperfusion via dampening NLRP3 inflammasome activity. Mol Immunol.
Applications
Undefined
Zhong, Z et al. (2018). New mitochondrial DNA synthesis enables NLRP3 inflammasome activation. Nature
Applications
WB, IB, PCA
Jabir et al. (2015). Mitochondrial damage contributes to Pseudomonas aeruginosa activation of the inflammasome and is downregulated by autophagy. Autophagy
Applications
IP, Co-IP
Shimada K et al. (2012). Oxidized mitochondrial DNA activates the NLRP3 inflammasome during apoptosis. Immunity
Applications
WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.