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5-mC Antibody
Mouse Monoclonal 33D3 IgG1
200-301-V02
100 µg
Liquid (sterile filtered)
ELISA, ChIP, Dot Blot
Plants, Vertebrates
Mouse
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Anti-5-mC (MOUSE) Monoclonal Antibody - 200-301-V02
5-methylcytosine, 5-methyl cytosine
Mouse
Monoclonal
IgG1
Target Details
Plants, Vertebrates
Other
Anti-5-mC Antibody was produced in mice by repeated immunization with 5-methylcytosine.
Anti-5-mC Antibody was purified was purified by gel filtration. Cross-reactivity with 5-mC from other sources has not been determined.
Application Details
ChIP, Dot Blot, ELISA
Anti-5-mC Antibody is tested for Dot Blotting, ELISA, Immunofluorescence, Methylated DNA Immunoprecipitation (MeDIP), and Sequencing (MeDIP-seq). Specific conditions for reactivity should be optimized by the end user.
Formulation
1.24 mg/ml by UV absorbance at 280 nm
0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2
0.05% (w/v) Sodium Azide
None
Shipping & Handling
Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
The 5-methylcytosine antibody (clone 33D3) is the most published and widely used antibody for DNA methylation analysis. Anti-5mC is ideal for research in Epigenetics due to its applicability to DNA methylation analysis.
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.