Rat DKK1 ELISA Kit
KOA0865
1 Kit
ELISA
Rat
46.9 pg/ml - 3000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Rat DKK1 AccuSignal ELISA Kit - KOA0865
Dickkopf 1, Dickkopf homolog 1, Dickkopf related protein 1, DKK 1, DKK1, DKK-1, hDkk 1, hDkk1, mDkk1, SK
ELISA Kit
46.9 pg/ml - 3000 pg/ml
Target Details
DKK1 - View All DKK1 Products
Rat
Expression system for standard: NSO; Immunogen sequence: S23-H270
Natural and recombinant rat DKK1. There is no detectable cross-reactivity with other relevant proteins.
D3Z9J1 - UniProtKB
NP_001099820.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 3000pg/ml, 1500pg/ml, 750pg/ml, 419.pg/ml, 187.5pg/ml, 93.8pg/ml, 46.9pg/ml rat DKK1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum or plasma (heparin, EDTA) to each empty well. We recommend that each rat DKK1 standard solution and each sample is measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Dickkopf-related protein 1 is a protein that in humans is encoded by the DKK1 gene, which was mapped to chromosome 10q11.2. This gene encodes a protein that is a member of the dickkopf family. It is a secreted protein with two cysteine rich regions and is involved in embryonic development through its inhibition of the WNT signaling pathway. Elevated levels of DKK1 in bone marrow, plasma and peripheral blood is associated with the presence of osteolytic bone lesions in patients with multiple myeloma.
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