Rat CXCL5 - LIX ELISA Kit
KOA0491
1 Kit
ELISA
Rat
15.6 pg/ml - 1000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Rat CXCL5/LIX AccuSignal ELISA Kit - KOA0491
AMCFII, C-X-C motif chemokine 5, chemokine (C-X-C motif) ligand 5, Cxcl5, CXCL5_HUMAN, ENA-78 (8-78), ENA-78(1-78), ENA-78(9-78), ENA78, Epithelial derived neutrophil activating peptide 78, Epithelial-derived neutrophil-activating protein 78, GCP2, Lipopolysaccharide-induced CXC chemokine, LIX, Neutrophil activating peptide ENA 78, neutrophil-activating protein 78, SCYB5, Scyb6, Small inducible cytokine B5, Small inducible cytokine subfamily B member 5, Small-inducible cytokine B5
ELISA Kit
15.6 pg/ml - 1000 pg/ml
Target Details
CXCL5 - View All CXCL5 Products
Rat
Expression system for standard: E.coli; Immunogen sequence: A38-Q130
Natural and recombinant rat CXCL5. There is no detectable cross-reactivity with other relevant proteins.
P97885 - UniProtKB
NP_071550.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml rat CXCL5 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of rat cell culture supernatants, serum, plasma(heparin, EDTA, citrate) or tissue homogenates to each empty well. It is recommended that each rat CXCL5 standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
C-X-C motif chemokine 5 is a protein that in humans encoded by the CXCL5 gene. The protein encoded by this gene, CXCL5 is a small cytokine belonging to the CXC chemokine family that is also known as epithelial-derived neutrophil-activating peptide 78(ENA-78). It is produced following stimulation of cells with the inflammatory cytokines interleukin-1or tumor necrosis factor-alpha. Expression of CXCL5 has also been observed in eosinophils, and can be inhibited with the type II interferon IFN-gamma. This chemokine stimulates the chemotaxis of neutrophils possessing angiogenic properties. It elicits these effects by interacting with the cell surface chemokine receptor CXCR2. The gene for CXCL5 is encoded on four exons and is located on human chromosome 4 amongst several other CXC chemokine genes. CXCL5 has been implicated in connective tissue remodeling. CXCL5 plays a role in reducing sensitivity to sunburn pain in some subjects, and is a potential target which can be utilized to understand more about pain in other inflammatory conditions like arthritis and cystitis.
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