Mouse Sclerostin - SOST ELISA Kit
KOA0679
1 Kit
ELISA
Mouse
15.6 pg/ml - 1000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Mouse Sclerostin/SOST AccuSignal ELISA Kit - KOA0679
BEER, CDD, Cortical hyperostosis with syndactyly, Sclerosteosis, Sclerostin, SOST, SOST_HUMAN, UNQ2976/PRO7455/PRO7476, VBCH
ELISA Kit
15.6 pg/ml - 1000 pg/ml
Target Details
SOST - View All SOST Products
Mouse
Expression system for standard: NSO; Immunogen sequence: Q24-Y211
Natural and recombinant mouse Sclerostin. There is no detectable cross-reactivity with other relevant proteins.
Q99P68 - UniProtKB
NP_077769.4 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml, 15.6pg/ml mouse Sclerostin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well.. It is recommended that each mouse Sclerostin standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
1 Kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Sclerostin is a protein that in humans is encoded by the SOST gene. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like(CTCK) domain and sequence similarity to the DAN(differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein(BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21
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