Mouse RANK ELISA Kit
KOA0497
1 Kit
ELISA
Mouse
62.5 pg/ml - 4000 pg/ml
Shipping info:
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Product Details
Mouse RANK AccuSignal ELISA Kit - KOA0497
CD265, FEO, LOH18CR1, Loss of heterozygosity 18 chromosomal region 1, ODFR, OFE, OPTB7, Osteoclast differentiation factor receptor, OSTS, PDB2, Receptor activator of NF-KB, Receptor activator of nuclear factor kappa B, TNFRSF11A, TNR11_HUMAN, TRANCER, Tumor necrosis factor receptor superfamily member 11A, Tumor necrosis factor receptor superfamily member 11a activator of NFKB, Tumor necrosis factor receptor superfamily member 11a NFKB activator
ELISA Kit
62.5 pg/ml - 4000 pg/ml
Target Details
TNFRSF11A - View All TNFRSF11A Products
Mouse
Expression system for standard: NSO; Immunogen sequence: Q30-P213
Natural and recombinant mouse RANK. There is no detectable cross-reactivity with other relevant proteins.
O35305 - UniProtKB
NP_033425.3 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 4000pg/ml, 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml mouse RANK standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse RANK standard solution and each sample be measured in duplicate.
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Receptor Activator of Nuclear Factor kappa B(RANK), also known as TRANCE Receptor, is a type I membrane protein that is expressed on the surface of osteoclasts and is involved in their activation upon ligand binding. RANK is a recently described TNF receptor family member, and its ligand, RANKL, promote survival of dendritic cells and differentiation of osteoclasts. RANK contains 383 amino acids in its intracellular domain(residues 234-616), which contain three putative TRAF-binding domains(termed I, II, and III). RANK interacts with various TRAFs through distinct motifs and activates NF-kappaB via a novel TRAF6 interaction motif, which then activates NIK, thus leading to NF-kappaB activation, whereas RANK most likely activates JNK through a TRAF2-interacting region in RANK.
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