Mouse MADCAM-1 ELISA Kit
KOA0801
1 Kit
ELISA
Mouse
125 pg/ml - 8000 pg/ml
Shipping info:
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Product Details
Mouse MADCAM-1 AccuSignal ELISA Kit - KOA0801
Addressin mucosal, hMAdCAM 1, hMAdCAM-1, MACAM1, MADCA_HUMAN, MAdCAM 1, MAdCAM-1, Madcam1, Mucosal addressin cell adhesion molecule 1, Mucosal vascular addressin cell adhesion molecule 1
ELISA Kit
125 pg/ml - 8000 pg/ml
Target Details
MADCAM1 - View All MADCAM1 Products
Mouse
Expression system for standard: NSO; Immunogen sequence: Q22-T365
Natural and recombinant mouse MADCAM-1. There is no detectable cross-reactivity with other relevant proteins.
Q61826 - UniProtKB
NP_038619.2 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 8000pg/ml, 4000pg/ml, 2000pg/ml, 1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml mouse MADCAM-1 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates or serum to each empty well. It is recommended that each mouse MADCAM-1 standard solution and each sample be measured in duplicate.
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
MADCAM1(Mucosal Vascular Addressin Cell Adhesion Molecule 1), also known as MACAM1, is a protein that is encoded by the MADCAM1 gene. By PCR-based analysis of somatic cell hybrids, Leung et al(1997) mapped the MACAM1 gene to chromosome 19. The protein encoded by this gene is an endothelil cell adhesion molecule that interacts preferentially with the leukocyte beta7 integrin LPAM-1(alpha4 / beta7), L-selectin, and VLA-4(alpha4/beta1) on myeloid cells to direct leukocytes into mucosal and inflamed tissues. It is a member of the immunoglobulin superfamily and is similar to ICAM-1 and VCAM-1.
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