Mouse Laminin ELISA Kit
KOA0245
1 Kit
ELISA
Mouse
156 pg/ml - 10000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Mouse Laminin AccuSignal ELISA Kit - KOA0245
Lamb2, LAMB2_HUMAN, LAMC1, LAMC2, LAMC3, laminin A, Laminin B, Laminin B1s chain, Laminin gamma 1, Laminin gamma 2, Laminin gamma 3, Laminin subunit beta-2, Laminin-11 subunit beta, Laminin-14 subunit beta, Laminin-15 subunit beta, Laminin-3 subunit beta, Laminin-4 subunit beta, Laminin-7 subunit beta, Laminin-9 subunit beta, OCCM, S-LAM beta, S-laminin subunit beta
ELISA Kit
156 pg/ml - 10000 pg/ml
Target Details
LAMA1; LAMA2; LAMB1; LAMB2 - View All LAMA1; LAMA2; LAMB1; LAMB2 Products
Mouse
from murine sarcoma basement membrane
Natural mouse Laminin. There is no detectable cross-reactivity with other relevant proteins.
Q61789 - UniProtKB
NP_034810.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 10000pg/ml, 5000pg/ml, 2500pg/ml, 1250pg/ml, 625pg/ml, 312pg/ml, 156pg/ml mouse Laminin standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Laminin standard solution and each sample be measured in duplicate.
Formulation
Heparin Sodium
1 Kit
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Laminin is a large basement membrane glycoprotein composed of three subunits designated the A, B1, and B2. Laminin has diverse biological functions, which include stimulating epithelial cell growth and differentiation. The nucleotide sequence of human laminin A chain has an open reading frame encoding 3075-amino acids. The human laminin A chain is at locus 18p11.3. The nucleotide sequence of the human laminin B1 reveals a 5358-base pair open reading frame that potentially codes for 1786 amino acids, including 20 amino acids of a presumptive signal peptide. The gene for the human laminin-B1 chain has been localized to chromosome 7, band q31. The B2 chain consists of six distinct domains, including two domains with alpha-helical, coiled-coil structures, two domains with cysteine-rich homologous repeats, and two globular domains. The amino acid sequences of the B2 and B1 chains demonstrate considerable homology.
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