Mouse IL-1 beta ELISA Kit standard curve.

Datasheet Protocol

Mouse IL-1 beta ELISA Kit

Name: Mouse IL-1 beta ELISA Kit
Brand: Rockland Immunochemicals
Price: 665.0 USD
Availability: OutOfStock
Rating: 5 (1 reviews)

1 References

Item No. KOA0211

Size: 1 Kit

Applications: ELISA

Reactivity: Mouse

Range: 12.5 pg/ml - 800 pg/ml

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Specifications for Mouse IL-1 beta ELISA Kit

Product Details

Description: Mouse IL-1 beta AccuSignal ELISA Kit - KOA0211

Synonyms: Catabolin, Hematopoietin 1, IFN beta inducing factor, IL 1, IL 1 beta, IL 1B, IL-1 beta, IL1, IL1 BETA, IL1B, IL1B_HUMAN, IL1F2, Interleukin 1 beta, Interleukin 1 beta precursor, Interleukin-1 beta, LAF, OAF, Osteoclast activating factor, Preinterleukin beta, Pro interleukin 1 beta

Detection Kit Type: ELISA Kit

Detection Range: 12.5 pg/ml - 800 pg/ml

Target Details

Gene Name: IL1B - View All IL1B Products

Reactivity: Mouse

Immunogen: Expression system for standard: E.coli; Immunogen sequence: V118-S269

Purity/Specificity: Natural and recombinant mouse IL-1 beta. There is no detectable cross-reactivity with other relevant proteins.

Database Links:

P10749 - UniProtKB

NP_032387.1 - NCBI Protein

Application Details

Tested Applications: ELISA

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 800pg/ml, 400pg/ml, 200pg/ml, 100pg/ml, 50pg/ml, 25pg/ml, 12.5pg/ml mouse IL-1 beta standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse cell culture supernatants, serum or plasma(heparin, EDTA) to each empty well. We recommend that each mouse IL-1 beta standard solution and each sample is measured in duplicate.

Formulation

Anticoagulant: Heparin Sodium

Concentration: 1 Kit

Shipping & Handling

Shipping Condition: Wet Ice

Storage Condition: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Expiration: See kit insert for complete instructions.

Background

Interleukin-1beta(IL-1beta) is a potent stimulator of bone resorption whose gene is mapped to 2q14, and has been implicated in the pathogenesis of high bone turnover and osteoporosis. IL-1beta, a prominent microglia-derived cytokine, caused oligodendrocyte death in coculture with astrocytes and microglia, but not in pure culture of oligodendrocytes alone1. It also can cause nuclear export of a specific NCOR corepressor complex, resulting in derepression of a specific subset of nuclear factor-kappa-B(NFKB)-regulated genes2. Furthermore, Microenvironmental IL-1beta and, to a lesser extent, IL-1alpha are required for in vivo angiogenesis and invasiveness of different tumor cells3. Additional, the cooperation of IL-1beta and PDGFB induces contractile-to-synthetic phenotype modulation of human aortic smooth muscle cells in culture4. Moreover, the association with disease may be explained by the biologic properties of IL-1beta, which is an important proinflammatory cytokine and a powerful inhibitor of gastric acid secretion.

References (1)

Zhang Q et al. (2021). Humanized anti‑TLR4 monoclonal antibody ameliorates lipopolysaccharide‑related acute kidney injury by inhibiting TLR4/NF‑κB signaling. Mol Med Rep.

Applications

EIA, ELISA

PubMed

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Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.