Mouse BMP-4 ELISA Kit
KOA0154
1 Kit
ELISA
Mouse
31.2 pg/ml - 2000 pg/ml
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Product Details
Mouse BMP-4 AccuSignal ELISA Kit - KOA0154
BMP 2B, BMP 4, BMP2B, BMP2B1, BOMPR4A, Bone morphogenetic protein 2B, Bone morphogenetic protein 4, DVR4, OTTMUSP00000021065, OTTMUSP00000021066, ZYME
ELISA Kit
31.2 pg/ml - 2000 pg/ml
Target Details
BMP-4 - View All BMP-4 Products
Mouse
Expression system for standard: NSO; Immunogen sequence: S293-R408
Natural and recombinant mouse BMP-4. There is no detectable cross-reactivity with other relevant proteins.
P21275 - UniProtKB
NP_001303289.1 - NCBI Protein
Application Details
ELISA
Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1ml per well of the 2000pg/ml,1000pg/ml, 500pg/ml, 250pg/ml, 125pg/ml, 62.5pg/ml, 31.2pg/ml mouse BMP-4 standard solutions into the precoated 96-well plate. Add 0.1ml of the sample diluent buffer into the control well (Zero well). Add 0.1ml of each properly diluted sample of mouse bone tissue, cell culture supernates or serum to each empty well. It is recommended that each mouse BMP-4 standard solution and each sample be measured in duplicate.
Shipping & Handling
Wet Ice
Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
See kit insert for complete instructions.
Bone morphogenetic protein 4 is a protein that in humans is encoded by the BMP4 gene which is located to 14q22-q23. The protein encoded by this gene is a member of the bone morphogenetic protein family which is part of the transforming growth factor-beta superfamily. BMP4 is a polypeptide belonging to the TGF-β superfamily of proteins. It, like other bone morphogenetic proteins, is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. It has been shown to be involved in muscle development, bone mineralization, and uteric bud development. BMP4 has also been implicated in Fibrodysplasia Ossificans Progressiva in which it is underexpressed. In human embryonic development, BMP4 is a critical signaling molecule required for the early differentiation of the embryo and establishing of a dorsal-ventral axis. BMP4 is secreted from the dorsal portion of the notochord, and it acts in concert with sonic hedgehog (released from the ventral portion of the notochord) to establish a dorsal-ventral axis for the differentiation of later structures. Also, BMP4 stimulates differentiation of overlying ectodermal tissue. Inhibition of the BMP4 signal (by chordin, noggin, or follistatin) causes the ectoderm to differentiate into the neural plate.
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